OLYMPUS 1-U2B230 Plan Oil Iris Uis Microscope Objective 50X /0.90

( Brand: Olympus ), ( Manufacturer Part Number: 1-U2B230 ), ( Part Type: Oil ), ( Country Of Origin: Japan )

Review OLYMPUS Plan 50x /0.90 Oil Iris Uis Microscope Objective

The **Olympus UPlanSApo 100x/1.35 Oil Iris UIS (UMPlanFL N.A. 1.35) Objective** is a high-performance, immersion-corrected microscope objective designed for researchers and professionals seeking unparalleled resolution, contrast, and optical clarity in fluorescence and brightfield imaging. Engineered with Olympus advanced **UPlanSApo** (Ultra Plan Super Apochromat) technology, this objective delivers exceptional correction across a broad spectrum of wavelengths, minimizing chromatic and spherical aberrations even at high magnifications. Its **1.35 numerical aperture (N.A.)** allows for superior light-gathering capability, enabling the visualization of fine structural details such as subcellular organelles, bacterial flagella, or intricate tissue morphology with remarkable precision. The **iris diaphragm** built into the design provides precise control over the working distance and illumination, enhancing contrast and reducing glare for sharper, more detailed images. Ideal for use with **UIS (Universal Infinity System) microscopes**, this objective is compatible with a wide range of fluorescence filters and high-resolution cameras, making it a versatile choice for applications in cell biology, microbiology, and materials science.

Constructed with **high-quality glass elements and anti-reflective coatings**, the UPlanSApo 100x/1.35 objective minimizes light scattering and maximizes transmission efficiency, ensuring vibrant, high-contrast images even when imaging thick or densely stained specimens. The **oil immersion** design significantly enhances resolution by reducing the refractive index mismatch between the objective and the cover glass, allowing for the visualization of features as small as **0.12 m** (theoretical resolution limit). The **0.17 mm working distance** requires careful specimen preparation but is compensated by the objective s exceptional depth of field and fine structural detail. Whether used in **fluorescence microscopy** for tracking fluorescently labeled proteins or in **brightfield imaging** for high-contrast differential interference contrast (DIC) applications, this objective excels in demanding research environments. Its robust construction and compatibility with **Olympus UIS infinity optics** make it a reliable long-term investment for laboratories prioritizing optical performance and durability.

The Olympus UIS Plan 50x/0.90 oil iris objective is a high-quality, specialized microscope objective designed for advanced imaging, particularly in fluorescence and high-resolution applications. Below is a detailed breakdown of its pros and cons, followed by a conclusion and recommendation.

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### **Pros of the Olympus UIS Plan 50x/0.90 Oil Iris Objective**

1. **High Numerical Aperture (NA 0.90)**

- The 0.90 NA provides superior light-gathering capability compared to lower NA objectives, resulting in finer resolution and better detail in imaging. This is particularly useful for observing fine cellular structures, fluorescence, and high-contrast samples.

2. **Iris Design**

- The iris mechanism allows manual adjustment of the aperture, which can improve contrast and resolution by controlling the amount of light entering the objective. This is especially beneficial for fluorescence microscopy, where controlling the excitation light is critical.

3. **UIS (Universal Infinity System) Compatibility**

- The UIS design is compatible with a wide range of Olympus infinity-corrected microscopes, making it versatile for various imaging setups. It also works well with digital cameras and advanced imaging systems.

4. **Plan-Apochromat Correction**

- The "Plan" designation indicates flat-field correction, reducing curvature of field errors and ensuring a sharp image across the entire field of view. The "Apochromat" correction minimizes chromatic aberration, providing better color accuracy and clarity.

5. **Oil Immersion for High Resolution**

- Oil immersion objectives are designed to minimize light scattering at the glass-air interface, maximizing resolution and contrast. The 50x magnification is ideal for detailed cellular or subcellular imaging.

6. **Durability and Build Quality**

- Olympus objectives are known for their robust construction, precise optics, and longevity. The 50x/0.90 is built to withstand heavy use in research and clinical settings.

7. **Compatibility with Advanced Techniques**

- This objective is well-suited for fluorescence microscopy, confocal microscopy, and other high-end imaging techniques where resolution and contrast are paramount.

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### **Cons of the Olympus UIS Plan 50x/0.90 Oil Iris Objective**

1. **High Cost**

- High-NA objectives, especially those with iris and apochromatic correction, are expensive. The 50x/0.90 is no exception, making it a significant investment for individuals or labs with limited budgets.

2. **Requires Oil Immersion**

- Oil immersion objectives necessitate the use of immersion oil between the objective and the slide. This adds complexity to sample preparation and cleanup, and the oil must be carefully managed to avoid contamination or drying out.

3. **Limited Working Distance**

- The 50x objective has a very short working distance (typically around 0.13 0.17 mm), which can make focusing difficult, especially with thick or uneven samples. This may require specialized sample preparation or coverslips.

4. **Sensitivity to Alignment**

- High-NA objectives are more sensitive to misalignment with the condenser or illumination system. Poor alignment can degrade performance, so precise setup is required.

5. **Not Suitable for Brightfield Only**

- While this objective works well for brightfield, its primary strengths lie in fluorescence and high-resolution applications. For general brightfield work, lower-magnification objectives may be more practical.

6. **Maintenance Requirements**

- Oil objectives require careful cleaning to avoid scratches or oil residue, which can degrade optical performance over time. This adds to the maintenance burden.

7. **Limited Field of View**

- At 50x magnification, the field of view is very small, which may not be ideal for observing larger samples or low-magnification surveys. Lower-magnification objectives are often used for initial sample inspection.

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### **Conclusion**

The Olympus UIS Plan 50x/0.90 oil iris objective is a powerful tool for advanced microscopy, particularly in research, clinical diagnostics, and high-resolution imaging applications. Its high numerical aperture, iris design, and apochromatic correction make it one of the best objectives for fluorescence and detailed cellular imaging. However, its high cost, requirement for oil immersion, and sensitivity to alignment and sample preparation can be significant drawbacks, especially for casual users or those working with limited budgets.

- Researchers in cell biology, microbiology, or pathology who require high-resolution imaging.

- Labs equipped with fluorescence or confocal microscopes.

- Users who prioritize image quality and are willing to invest in premium optics.

For users who primarily need brightfield imaging or work with a variety of sample types, a lower-magnification objective (e.g., 40x or 60x) might be more practical. Additionally, those on a tight budget may consider alternatives from other manufacturers (e.g., Zeiss, Nikon) or used objectives, though quality may vary.

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### **Recommendation**

- You are working in a field that demands high-resolution imaging (e.g., fluorescence microscopy, confocal microscopy, or detailed cellular analysis).

- Your budget allows for the investment in a premium objective, as it will significantly enhance your imaging capabilities.

- You are already using an Olympus UIS microscope system and need a high-end objective for specialized applications.

- You are prepared to handle the maintenance and alignment requirements associated with a high-NA oil objective.

- You primarily use brightfield microscopy and do not require high magnification.

- Your budget is limited, and you can achieve similar results with a lower-cost alternative (e.g., a 60x/1.40 oil objective, which may offer better value for some applications).

- You lack experience with oil immersion objectives or do not have the infrastructure to maintain them properly.

- Your samples are thick or uneven, as the short working distance may make focusing difficult.

For most advanced microscopy applications, this objective is an excellent choice, but it is not a "one-size-fits-all" solution. Carefully assess your needs and budget before purchasing.

Details:

OLYMPUS PLAN 50X /0.90 OIL, WITH IRIS, UIS 2, MICROSCOPE OBJECTIVE. IRIS 0.5 - 0.90 SMOOTH MOVEMENT.

part #: 1u2b230 price

• $995.00

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