LEICA Resonant Scanning Tcs Microscope 15-30961-013 SP5

( Brand: Leica ), ( Manufacturer Part Number: 1530961013 ), ( Model: 15-30961-013 ), ( Country Of Origin: Germany )

Review LEICA 30961 013 Resonant Scanning System Tcs Sp5 Microscope

The **Leica TCS SP5 Resonant Scanning Confocal Microscope (Model 1530961013 / 1530961013)** represents the pinnacle of optical imaging technology, engineered to deliver unparalleled precision, speed, and versatility for advanced biological and materials research. This state-of-the-art microscope combines resonant scanning technology with Leica s renowned optical engineering to achieve exceptional performance in live-cell imaging, high-resolution fluorescence microscopy, and multiphoton excitation. At its core, the resonant scanner enables rapid, high-speed acquisition with minimal drift and superior image stability, making it ideal for capturing dynamic biological processes in real time without compromising spatial resolution. The system is equipped with a **dual laser launch** and **acousto-optical beam splitters (AOBS)**, allowing seamless switching between multiple excitation wavelengths from ultraviolet to near-infrared while maintaining high efficiency and minimal cross-talk. This flexibility is further enhanced by the **HyD hybrid detectors**, which combine the sensitivity of photomultiplier tubes (PMTs) with the speed of avalanche photodiodes (APDs), ensuring optimal signal detection across a broad spectrum of fluorophores, including quantum dots and fluorescent proteins.

The TCS SP5 s modular design accommodates a wide array of objectives, from **high-numerical-aperture (NA) oil immersion lenses** for submicron resolution to **water-immersion objectives** for extended live-cell imaging, all optimized for minimal spherical aberration and maximum light transmission. The microscope s **galvanometric and resonant scanning modes** provide researchers with the ability to balance speed and resolution, with resonant scanning delivering frame rates exceeding **100 Hz** while maintaining diffraction-limited performance. This capability is particularly advantageous for tracking fast-moving cellular structures, such as mitochondria, vesicles, or calcium dynamics, without the need for compromising spatial resolution. Additionally, the **multiphoton excitation module** enables deep-tissue imaging with reduced phototoxicity, making it an invaluable tool for studying thick biological samples, such as brain slices or tissue-engineered constructs, where traditional confocal microscopy would suffer from scattering and autofluorescence.

Beyond its imaging prowess, the TCS SP5 is equipped with **Leica s advanced software suite**, including **LAS AF** (Leica Application Suite Advanced Fluorescence), which offers intuitive control over acquisition parameters, automated sample staging, and sophisticated image processing tools. Features such as **FRAP (Fluorescence Recovery After Photobleaching), FRET (F rster Resonance Energy Transfer), and spectral imaging** allow researchers to investigate molecular interactions, protein dynamics, and energy transfer mechanisms with high precision. The system also supports **time-lapse imaging** with precise environmental control, including **incubators, CO control, and temperature stabilization**, ensuring optimal conditions for long-term live-cell experiments. For applications requiring even greater sensitivity, the **Leica Lightning** module can be integrated, enhancing image quality by reducing noise and improving contrast without sacrificing resolution a particularly useful feature for low-fluorescence samples.

The Leica TCS SP5 is not merely an imaging tool but a **research platform** designed to push the boundaries of biological discovery. Its combination of **high-speed resonant scanning, multiphoton capability, and hybrid detection** makes it suitable for a diverse range of applications, from **neuroscience and cell biology** to **materials science and pharmaceutical research**. Whether used for **high-content screening, super-resolution imaging (when paired with STED or SOFI techniques), or complex multiparameter experiments**, this microscope provides the robustness and flexibility required to tackle some of the most challenging questions in modern science. With its **rugged, modular construction and Leica s reputation for reliability**, the TCS SP5 stands as a cornerstone in any advanced microscopy laboratory, empowering researchers to visualize the unseen with clarity, speed, and unmatched precision.

### **Pros and Cons of buying a Leica TCS SP5 Resonant Scanning Confocal Microscope (Model 1530961013 / 1530961013-013)**

#### **Pros**

1. **Superior Image Resolution and Clarity**

The TCS SP5 is a high-end confocal microscope equipped with resonant scanning technology, which significantly enhances imaging speed and resolution. This is particularly valuable for live-cell imaging, fluorescence studies, and detailed cellular or subcellular analysis. The system supports multi-photon excitation, allowing deeper tissue penetration and reduced phototoxicity.

2. **Advanced Fluorescence Capabilities**

The microscope features multiple laser lines (UV, visible, and near-infrared) and high-sensitivity detectors, making it suitable for a wide range of fluorescence applications, including FRET (F rster Resonance Energy Transfer), FRAP (Fluorescence Recovery After Photobleaching), and lifetime imaging. This versatility is ideal for research in cell biology, neuroscience, and molecular imaging.

3. **High-Speed Imaging and Live-Cell Applications**

Resonant scanning dramatically increases frame rates compared to traditional galvanometric scanners, enabling real-time imaging of dynamic cellular processes. This is crucial for studying fast biological phenomena such as calcium signaling, organelle movement, or neuronal activity.

4. **Modular and Upgradable Design**

The TCS SP5 is part of Leica s modular microscope platform, meaning it can be integrated with additional components such as environmental control chambers, high-content screening systems, or advanced detectors (e.g., HyD detectors for superior sensitivity). This scalability ensures the system can evolve with research needs.

5. **Robust Software Suite**

Leica s LAS AF (Advanced Fluorescence) software provides powerful image acquisition, processing, and analysis tools. Features such as deconvolution, 3D reconstruction, and automated quantification enhance productivity and data quality. The software is user-friendly yet powerful enough for complex experiments.

6. **Precision and Reliability**

Leica is a well-established brand known for precision optics and mechanical stability. The TCS SP5 is built with high-quality components, reducing maintenance requirements and ensuring long-term reliability. The resonant scanner is particularly durable, minimizing wear compared to traditional scanners.

7. **Compatibility with Advanced Techniques**

The microscope supports advanced imaging modalities such as STED (Stimulated Emission Depletion) microscopy when paired with appropriate modules, enabling super-resolution imaging beyond the diffraction limit. This is invaluable for studying fine structural details in biology.

8. **Excellent Customer Support and Service**

Leica offers comprehensive technical support, training, and service networks, which are critical for maintaining high-performance microscopy. Many institutions and researchers rely on Leica s expertise for troubleshooting and system optimization.

---

#### **Cons**

1. **High Initial Cost**

The TCS SP5 is an expensive investment, with the base model and additional modules (e.g., multi-photon, environmental chambers) pushing the price into the high six or low seven figures. This can be a significant barrier for smaller labs, startups, or institutions with limited budgets.

2. **Complexity and Steep Learning Curve**

The advanced features of the TCS SP5 require trained personnel to operate effectively. Researchers or technicians must undergo extensive training to fully utilize the microscope s capabilities, which can slow down initial productivity. The software and imaging techniques (e.g., FRET, FRAP) also demand expertise.

3. **Maintenance and Operational Costs**

While Leica microscopes are durable, they require regular maintenance, calibration, and occasional part replacements. Consumables such as lasers, filters, and detectors may need periodic upgrades, adding to long-term operational expenses. The resonant scanner, though robust, may still require occasional servicing.

4. **Space and Infrastructure Requirements**

The TCS SP5 is a large, specialized instrument that requires a dedicated, stable environment (e.g., temperature-controlled room, vibration isolation, and proper ventilation). Setting up the microscope may involve significant infrastructure investments, such as upgrading lab facilities or installing specialized tables.

5. **Depreciation Over Time**

High-end microscopes like the TCS SP5 may depreciate rapidly, especially as newer models with improved features (e.g., AI-assisted imaging, faster resonant scanners) are introduced. Institutions must weigh the long-term value of the investment against emerging technologies.

6. **Limited Accessibility for Non-Experts**

The complexity of the system may restrict its use to trained operators, limiting accessibility for collaborative or interdisciplinary research. Junior researchers or non-specialists may struggle to perform advanced imaging without supervision.

7. **Potential for Over-Specification**

Depending on the research focus, some labs may find that the TCS SP5 offers more capabilities than strictly necessary. For example, a lab primarily focused on brightfield or phase-contrast imaging might not fully utilize the confocal and fluorescence features, making the investment less justified.

8. **Dependency on Leica s Ecosystem**

Users are somewhat locked into Leica s hardware and software ecosystem. Switching to alternative systems (e.g., Zeiss, Nikon) later may require retraining and compatibility issues, particularly if proprietary data formats or workflows are involved.

---

### **Conclusion**

The Leica TCS SP5 Resonant Scanning Confocal Microscope is one of the most advanced and capable confocal systems available, offering unmatched resolution, speed, and versatility for fluorescence imaging. It is an ideal choice for research-intensive environments where live-cell imaging, multi-photon microscopy, and advanced fluorescence techniques are critical. The investment is justified for institutions with substantial funding, experienced personnel, and a need for cutting-edge imaging capabilities.

However, the high cost, complexity, and maintenance requirements make it less suitable for smaller labs, budget-conscious institutions, or those with less specialized research needs. The decision to purchase should be based on a thorough assessment of the lab s current and future requirements, budget constraints, and the availability of trained staff.

### **Recommendation**

- Your research heavily relies on high-resolution fluorescence imaging, live-cell dynamics, or advanced techniques like FRET/FRAP.

- You have a dedicated budget for a premium microscope and can justify the long-term value.

- Your lab has or can train skilled personnel to operate and maintain the system.

- You require a modular, future-proof instrument that can adapt to evolving research needs.

- Your budget is limited, and a mid-range confocal microscope (e.g., Leica SP8, Zeiss LSM 880) would suffice for your applications.

- Your research does not require the highest imaging speeds or super-resolution capabilities.

- You lack the infrastructure (e.g., stable environment, space) to support such a high-end system.

- You prefer a more user-friendly or cost-effective option, such as a spinning-disk confocal microscope for live-cell imaging.

For labs that cannot afford the TCS SP5 but still need high-performance confocal imaging, exploring used or refurbished models (with proper warranty and support) or leasing options may be a viable compromise. Additionally, consulting with colleagues or core facility directors who have experience with the TCS SP5 can provide practical insights into its daily usability and long-term value.

Details:

Part number 15 30961 013, Pulled from a surplus Leica TCS SP5 microscope system. Good used condition - Please see pictures 100% guaranteed to arrive in good working order.

part #: 1530961013 price

• $1499.00

specifications microscopepartzo:

• brand: Leica
• mpn: 1530961013
• model: 15-30961-013
• country of origin: Germany

general microscopepartzo:

• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories
• condition: Used

seller microscopepartzo:

• rating: 100.0%
• city: Gainesville, Florida
• # reviews: 1884

offer microscopepartzo:

• started: March 25, 2026
• options: Ship-to-home
• quantity: 1
• best offers: True
• sold: 0
• availability: In Stock

shipping microscopepartzo:

• class: Standard
• transit: 3-9 Days
• type: Fixed
• cost: $0.00
• service: Standard

payments microscopepartzo:

• wallet: Google-pay
• credit card: Visa
• other

returns microscopepartzo:

• policy: Returns Accepted
• time: 30 Days
• paid by: Seller
• method: Money back

Leica 1530961013 Is Similar To:

• Microscope Plan Long-barrel 1x Achromat 0.025 Iris Infinity Leica Objective (13.5% similar) Make sure your microscope will truly accommodates very long lens that measures 55MM vertically. 706, conforming to the RMS standard, with aperture iris, and is infinity corrected. The objective has a mounting thread 20. Leica 1x plan achromatic iris infinity long barrel microscope objective Leica 1x...(January 25th, 2015)
• Leica Microscope Tube Photo 0.66x mount 2 35mm Trinocular Efocus Adapter Port (21.2% similar) This adapter is suited for 1/2 and 1/3 image sensor chips microscope picture using is capture. 66X Leica ex focus microscope camera port para focal adapter to 35MM photo tube c mount. The head attached to a modified microscope base. The device is designed to insert to any microscope which contains a...(January 26th, 2015)
• 4x Parts 0.67x Head Leica Block Microscope Gz6 Focus Repair (29.9% similar) ...(February 12th, 2015)
• 0.502.29553 Leica New Microscope Pressure Plate (30% similar) O microscope pressure plate b: RP inventory location: MF3 6-2 this offer is for a new Leica. A non-paying bidder case will automatically be initiated after this time. Please prior to sale for an accurate shipping quote. A credit card purchase is only shipped when the money transferred from ...(November 20th, 2018)
• 020-507.010 Leitz Leica Biomed Microscope Includes Objectives (28.4% similar) Leica LTZ boomed. 90 condenser eff LTZ OBJECTIVE4. 17 eff LTZ OBJECTIVE10 microscope wit Periclean 10X 18 eyepiece objective includes everything you see in the listing. 17 eff LTZ OBJECTIVE40 oil microscope pair of 10X 18 Periclean eyepieces stage with A0. You are buying on a working great condition...(April 2nd, 2018)
• 020-654.092-000 Leica Microsystems Microscope Beamsplitter (36.6% similar) Leica micro systems microscope misapplied...(July 18th, 2017)
• 090-132.103 Leica Dm Irb Microscope Frame Sold For Parts As Is (28.1% similar) This is my: a Leica Dem orb microscope frame sold for parts as. Pin to plug into the power supply are bent and receptacle is missing gone. Cases count against me even if they are resolved. Didn't try to power this up. Sold as is for parts. I am not overjoyed with whats happening, but have to roll it...(April 8th, 2018)
• 373450 Camera Heerbrugg Wild Leica Microscope 093-032.010 Film Leitz (22.7% similar) 010 film back cosmetic 8 as pictured. You are purchasing exactly what you see in the pictures. Glass optic clean glass with some minor cleaning marks. Mechanical problem none offer includes LTZ microscope camera...(February 9th, 2015)
• Adapter 0.5x Leica 1.0xlens Leitz Microscope 3 2 Camera Iso38 Olympus Nikon M4 (30.5% similar) Assembly pieces minus. The adapter uses a standard ISO 38MM x c mount LTZ Leica wild ISO 38. The device is designed to insert into any microscope which contains a 38MM photo port bottom lower portion of picture. 0x port adapter see picture below for separate parts. 0x with f mount. 54 5, 31, 6, 49 ...(February 13th, 2015)
• 10246634 Leica Wild 246634 Coaxial Microscope Illuminator (30.8% similar) 25X when installed. I will describe the item best as can based on visual inspection and my knowledge of. This unit is in excellent cosmetic and working condition has been tested on my microscope. The wild coaxial Illuminati has a magnification factor of a lamp is included. Cosmetics are clean and ...(February 26th, 2018)
• 10382162 Leica Microscope Objective Lens (28.3% similar) Priced for fast sale. 50 for continental USA. You are buying on a Leica microscope objective lens f 200MMPART 10382162THE is in new condition. Shipping and handling 18...(April 6th, 2018)