OLYMPUS 325990 Nic Phase Contrast Lwdnc Microscope 0.55 IMT-2

( Brand: Olympus ), ( Manufacturer Part Number: 325990 ), ( Model: 0.55 ), ( Country Of Origin: Japan )

Review OLYMPUS Nic Phase Contrast Lwdnc Microscope

The **Olympus U-DAWN 325990 NIC Phase Contrast Low Working Distance (LWD) Condenser (NA 0.55)** is a high-performance optical component meticulously engineered to elevate the imaging capabilities of the **IMT-2 inverted microscope**, delivering unparalleled clarity and precision for phase contrast microscopy. Designed with advanced optical engineering, this condenser features a **low working distance (LWD) of 0.55 NA**, allowing researchers to observe live cell cultures, thin tissue sections, and delicate biological specimens with exceptional detail while minimizing sample disturbance. The **NIC (Nomarski Interference Contrast) phase contrast mechanism** enhances the visualization of transparent and unstained specimens by converting phase shifts in light waves into visible contrast, making subtle structural differences such as cellular organelles, membrane dynamics, and fine intracellular details distinctly observable. This condenser is particularly well-suited for applications in cell biology, microbiology, and developmental studies, where the ability to capture high-resolution images of live, unstained samples is critical. Constructed with durable, precision-ground glass optics and a robust metallic housing, the U-DAWN condenser ensures long-term stability and consistent performance, even under demanding laboratory conditions. Its compatibility with the **IMT-2 inverted microscope** further extends its utility, offering researchers a seamless integration of advanced imaging technology into their workflows. Whether used for dynamic live-cell imaging, structural analysis, or educational demonstrations, this condenser exemplifies Olympus commitment to innovation in microscopy, providing a reliable tool for advancing biological research with clarity and precision.

The Olympus U-DAPlanApo 325990 (NIC Phase Contrast, LWD, NA 0.55) objective is a high-quality phase contrast lens designed for use with the IMT-2 inverted microscope. Below is a detailed breakdown of its pros and cons, followed by a conclusion and recommendation.

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1. **High-Quality Phase Contrast Imaging**

The objective is optimized for phase contrast microscopy, which enhances the visibility of transparent or thin specimens by converting phase shifts in light waves into visible contrast. This is particularly useful for observing live cells, thin tissue sections, and other transparent biological samples.

2. **Long Working Distance (LWD)**

With a working distance of 0.55 mm, this objective allows for greater flexibility when working with thicker coverslips or specimens that require more space between the objective and the slide. This reduces the risk of damaging delicate samples or coverslips during observation.

3. **Numerical Aperture (NA 0.55)**

The moderate numerical aperture provides a good balance between resolution and depth of field. While not as high as oil-immersion objectives (e.g., NA 1.25 1.4), it offers sufficient resolution for many routine biological applications without requiring immersion oil, which simplifies sample preparation.

4. **Compatibility with Olympus IMT-2 Microscope**

The objective is specifically designed for the Olympus IMT-2 inverted microscope, ensuring optimal performance and alignment with the microscope s optical system. This reduces the risk of compatibility issues or poor image quality.

5. **Durability and Precision**

Olympus objectives are known for their precision engineering and durability. The U-DAPlanApo series is designed to maintain consistent optical performance over time, even with frequent use.

6. **Wide Field of View**

Phase contrast objectives like this one typically offer a broader field of view compared to high-magnification oil-immersion lenses, making them suitable for observing larger areas of a sample at once.

7. **Non-Centric (NIC) Design**

The "NIC" (Non-Centric) phase ring design is less sensitive to misalignment of the phase ring relative to the objective, which can be advantageous in settings where precise centering is challenging.

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1. **Limited Resolution Compared to Higher NA Objectives**

While NA 0.55 provides good contrast and depth of field, it does not offer the same level of resolution as higher NA objectives (e.g., NA 1.25 1.4 with oil immersion). For detailed structural studies (e.g., fine cellular organelles), a higher NA objective may be necessary.

2. **No Oil Immersion**

Since this is a dry objective (no oil immersion), it lacks the higher resolution and contrast achievable with oil-immersion objectives. This may limit its use in applications requiring ultra-high magnification or fine detail.

3. **Potential for Light Loss**

Phase contrast objectives can introduce some light loss due to the phase ring and annular diaphragm, which may require brighter illumination or longer exposure times in photography.

4. **Working Distance Trade-Off**

While the 0.55 mm working distance is beneficial for thicker samples, it may feel restrictive compared to objectives with longer working distances (e.g., 1.0 mm or more). This could limit the types of samples that can be observed without adjusting the slide position.

5. **Cost Relative to Lower-End Objectives**

Although not excessively expensive, this objective is more costly than basic dry objectives (e.g., NA 0.10 0.25). For basic phase contrast work, a lower-cost alternative might suffice.

6. **Maintenance Sensitivity**

Phase contrast objectives require careful alignment of the phase ring and condenser diaphragm. Misalignment can degrade image quality, and adjustments may be needed periodically, which could be a drawback in less controlled environments.

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The Olympus U-DAPlanApo 325990 (NIC Phase Contrast, LWD, NA 0.55) is an excellent choice for applications requiring high-quality phase contrast imaging with a balance of resolution, working distance, and ease of use. It is particularly well-suited for observing live cells, thin tissue sections, or samples where a longer working distance is advantageous. However, its moderate numerical aperture and lack of oil immersion may limit its use in high-resolution imaging tasks. If your primary goal is to observe transparent or semi-transparent samples with good contrast and a reasonable field of view, this objective will perform reliably. For applications demanding ultra-high resolution (e.g., detailed organelle visualization), a higher NA oil-immersion objective would be more appropriate.

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- You require phase contrast microscopy for routine biological observations (e.g., cell culture, thin tissue sections).

- You need a longer working distance to accommodate thicker coverslips or samples.

- You are working exclusively with the Olympus IMT-2 microscope and want optimal compatibility.

- You prioritize contrast and depth of field over absolute maximum resolution.

- You frequently work with samples requiring ultra-high resolution.

- Your budget is constrained, and you can achieve similar results with a less expensive objective.

- You anticipate needing a wider range of magnifications or immersion capabilities in the future.

Overall, this objective is a solid investment for phase contrast microscopy on the IMT-2, provided your needs align with its strengths.

Details:

The bottom glass/ lens piece shows what looks like water stains on. Olympus NIC / PHASE CONTRAST LWDNC 0.55 for IMT-2 Microscope, The body is in good cosmetic condition. See Pics, Filters settings: 0, 10PC, 40PC, 10 NIC, 20 40NIC. Unable to remove with a standard glass cleaner.

part #: 325990 prices

• $245.00-$1013.40

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• brand: Olympus
• mpn: 325990
• model: 0.55
• country of origin: Japan

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• started: June 10, 2026

general microscopepartzo:

• condition: Used
• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories

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