
( Brand: Chroma ), ( Manufacturer Part Number: 41020 )
The HQ480-20x Chroma 41020 Narrow-Band eGFP Minimizing Autofluorescence HQ510 Filter Set is a high-quality, specialized optics solution designed for advanced fluorescence microscopy applications. This filter set is optimized for excitation and emission of enhanced green fluorescent protein (eGFP) while minimizing the interference of unwanted autofluorescence.
The set includes a HQ480/20x excitation filter that allows for the efficient excitation of eGFP with minimal spillover into other fluorophores. The HQ510 LP emission filter, on the other hand, effectively blocks excitation light and passes the emitted fluorescence from eGFP, ensuring high signal-to-noise ratio and improved image quality.
The 41020 narrow-band emission filter set further enhances the specificity of the eGFP detection by significantly reducing the transmission of light outside of the eGFP emission spectrum, thus minimizing the interference from autofluorescence. This feature is particularly useful in applications where low background fluorescence is essential for accurate and precise data acquisition.
This filter set is suitable for various microscopy applications, including live cell imaging, fluorescence microscopy, and fluorescence-activated cell sorting (FACS). With its high-quality components and optimized design, the HQ480-20x Chroma 41020 Narrow-Band eGFP Minimizing Autofluorescence HQ510 Filter Set provides researchers with a powerful tool for studying eGFP-labeled samples with enhanced sensitivity and specificity.
Product: HQ480-20x Chroma 41020 Narrow-band EGFP, HQ510
Pros:1. Low Autofluorescence: This product is designed to minimize autofluorescence, which can interfere with fluorescence microscopy. This feature allows for a clearer and more accurate observation of the sample.
2. Narrow-band EGFP: The narrow-band filter specific to EGFP (Enhanced Green Fluorescent Protein) ensures a specific and intense emission of green fluorescence from the sample, enhancing the contrast and detail.
3. High Quality: The HQ480 and HQ510 filters are known for their high quality, providing excellent transmission and blocking properties.
4. Chroma: Chroma is a reputable brand in the field of optical filters, ensuring a reliable and durable product.
Cons:1. Cost: High-quality optical filters like the HQ480-20x Chroma 41020 Narrow-band EGFP, HQ510 can be expensive, which may be a limiting factor for some users.
2. Specificity: The filter is designed for EGFP, so it may not be suitable for other fluorescent proteins or samples that do not emit green fluorescence.
3. Compatibility: Ensure the filter is compatible with your specific microscope and imaging system to avoid any potential issues.
Conclusion:The HQ480-20x Chroma 41020 Narrow-band EGFP, HQ510 is a high-quality optical filter designed to minimize autofluorescence and enhance the observation of samples expressing EGFP. While it may be expensive, its specificity, high quality, and low autofluorescence make it a valuable tool for fluorescence microscopy. If you are working with EGFP samples and require a high-quality filter, this product is recommended. However, ensure it is compatible with your specific microscope and imaging system before purchase.
Calipers in the picture is not included. The hq480 20x and HQ510 20m are 25mm in diameter.
I'm guessing that this would go into a Zeus's reflector module fl. Cases count against me even if they are resolved.
I am not overjoyed with whats happening, but have to roll it.
Note that the hq480 20x has some mottling on filter. Don't know if this is normal.
See the pictures.