ZEISS 440761 Plan Apochromat Oil Microscope Objective 63X/1.40 /0.17

( Brand: Zeiss ), ( Manufacturer Part Number: 440761 ), ( Model: 440761 03 63X/1.40 /0.17 ), ( Part Type: Oil ), ( Country Of Origin: Germany ), ( Intended Use/discipline: Biological Laboratory, Medical Laboratory )

Review ZEISS Plan apochromat 63x/1.40 Oil /0.17 Microscope Objective

The **Zeiss Plan-Apochromat 63x/1.40 Oil Immersion Objective (440761)** represents the pinnacle of optical engineering, meticulously crafted to deliver unparalleled resolution, contrast, and precision in high-magnification microscopy. Engineered with Zeiss s signature precision, this objective combines a **numerical aperture of 1.40** with a **corrected working distance of 0.17 mm**, enabling it to harness the full potential of oil immersion for maximum light gathering and image clarity. The **Plan-Apochromat** design ensures exceptional flat-field performance across the entire field of view, minimizing curvature of field and chromatic aberrations critical for applications requiring flawless imaging of fine structural details, such as cellular organelles, fluorescent markers, or high-contrast specimens. Constructed with **high-index glass elements and multi-layer coatings**, this objective minimizes spherical and chromatic aberrations, delivering sharp, distortion-free images with minimal color fringing, even at the edges of the field. Its **immersion oil compatibility** (refractive index 1.515) optimizes light transmission, enhancing contrast and resolution to reveal sub-micron details with remarkable fidelity. The robust, durable construction ensures stability under high magnification, making it ideal for demanding applications in life sciences, materials research, and advanced fluorescence imaging. Whether used in brightfield, phase contrast, or fluorescence microscopy, the **Zeiss Plan-Apochromat 63x/1.40** objective sets a new standard for optical performance, empowering researchers to explore the finest details of their specimens with confidence.

### **Pros and Cons of the Zeiss Plan-Apochromat 63x/1.40 Oil Immersion Objective (440761)**

#### **Pros**

1. **Exceptional Resolution and Clarity**

The 63x magnification with a numerical aperture (NA) of 1.40 delivers superior optical performance, making it ideal for high-resolution imaging of fine cellular structures, fluorescence microscopy, and detailed fluorescence tagging. The apochromatic correction minimizes chromatic aberration, ensuring sharp, distortion-free images across multiple wavelengths.

2. **High Numerical Aperture (NA 1.40)**

A high NA objective maximizes light gathering, improving contrast and resolution, which is critical for observing thin sections of biological specimens, such as thin tissue slices or subcellular components like organelles.

3. **Plan-Apochromat Design**

The "plan" correction ensures a flat field of view, reducing curvature of field errors that can distort images at high magnification. This is particularly useful for wide-field imaging and photomicrography where a uniform focus plane is essential.

4. **Compatibility with Fluorescence Microscopy**

The objective is optimized for fluorescence applications, with minimal autofluorescence and high transmission efficiency. It works well with common fluorophores (e.g., FITC, Texas Red, DAPI) and is suitable for confocal and super-resolution techniques when paired with appropriate illumination.

5. **Durability and Build Quality**

Zeiss objectives are known for their robust construction, precision engineering, and long-term stability. The 63x oil immersion objective is built to withstand frequent use in demanding laboratory environments.

6. **Wide Range of Applications**

Beyond fluorescence, it can be used for brightfield, phase contrast, and differential interference contrast (DIC) microscopy, though oil immersion is typically required for optimal performance in these modes.

7. **Compatibility with Modern Microscopes**

It is designed to work seamlessly with most high-end inverted and upright microscopes equipped with oil immersion condensers and appropriate illumination sources (e.g., mercury or LED lamps for fluorescence).

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#### **Cons**

1. **High Cost**

The Zeiss Plan-Apochromat 63x/1.40 is one of the most expensive objectives available, often costing several thousand dollars. This makes it a significant investment, especially for laboratories or individual researchers on a tight budget.

2. **Requires Oil Immersion**

Achieving the full NA of 1.40 necessitates the use of immersion oil, which adds complexity to sample preparation and maintenance. Improper oil application can damage the objective or reduce performance. Additionally, oil can degrade over time, requiring frequent replacement.

3. **Limited Working Distance**

The working distance is very short (typically around 0.17 mm), which can make sample preparation and focusing challenging. This is particularly problematic for thick specimens or when using coverslips of varying thickness. A coverslip thickness of 170 m is recommended for optimal performance.

4. **Sensitivity to Sample Preparation**

High-NA objectives are sensitive to the quality of the sample mount. Air bubbles, uneven coverslip thickness, or improper oil application can introduce aberrations and reduce image quality. This requires meticulous sample preparation skills.

5. **Maintenance and Cleaning Challenges**

The high magnification and NA make the objective prone to smudging and contamination from oil, dust, or sample debris. Cleaning requires careful handling to avoid scratching the lens surfaces, and improper cleaning can void the warranty.

6. **Not Suitable for Low-Magnification Work**

While versatile, this objective is optimized for high magnification and is not practical for lower magnification tasks (e.g., 10x or 20x). Laboratories needing a wide range of magnifications may require additional objectives.

7. **Potential for Aberrations in Non-Optimal Conditions**

If the microscope setup is not properly aligned (e.g., incorrect condenser NA, misaligned illumination), aberrations such as spherical or coma aberrations can degrade image quality. This requires precise calibration of the microscope system.

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### **Conclusion**

The Zeiss Plan-Apochromat 63x/1.40 oil immersion objective is one of the finest high-magnification objectives available, offering unmatched resolution, clarity, and versatility for advanced fluorescence and high-resolution microscopy. Its apochromatic and plan-corrected design ensures superior image quality, making it indispensable for researchers studying fine cellular details, fluorescence labeling, and super-resolution techniques.

However, its high cost, requirement for meticulous sample preparation, and sensitivity to maintenance make it less accessible for casual users or laboratories with limited budgets. It is best suited for professional research settings where high-resolution imaging is critical and where the investment can be justified by the quality of results.

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### **Recommendation**

- You are working in a research environment where high-resolution imaging is essential (e.g., cell biology, neuroscience, molecular biology).

- Your budget allows for the investment, and the objective will be used frequently enough to justify its cost.

- Your microscope setup is compatible with oil immersion and properly aligned for high-NA objectives.

- You have access to trained personnel or resources to handle sample preparation, maintenance, and cleaning.

- Your budget is limited; lower-cost objectives from other manufacturers (e.g., Nikon Plan Apo, Olympus UPlanSApo) may offer comparable performance at a reduced price.

- You do not require fluorescence microscopy; a lower-magnification objective (e.g., 40x) may suffice for your needs.

- Your samples are thick or difficult to prepare; a lower-NA objective (e.g., 60x/1.4) or a water-immersion objective (if oil is impractical) might be more suitable.

- You are a student or casual user; renting or borrowing the objective from a core facility may be a more cost-effective solution.

For most professional research laboratories focused on high-end microscopy, the Zeiss Plan-Apochromat 63x/1.40 remains a top-tier choice, offering a balance of performance, durability, and optical excellence. However, weigh the pros and cons carefully against your specific needs and resources before making a purchase.

Details:

I'm not a microscope dealer or expert. Includes the following microscope objective: 1 - ZEISS 440761 03 PLAN-APOCHROMAT 63X/1.40 OIL /0.17, This may need to be cleaned. I looked at the lens with an eyepiece and did not see any lamination, cracks or scratches on.

part #: 440761 prices

• $999.98-$1499.99

specifications microscopepartzo:

• brand: Zeiss
• mpn: 440761
• model: 440761 03 63x/1.40 /0.17
• part type: Oil
• country of origin: Germany
• intended use/discipline: Biological Laboratory, Medical Laboratory

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• # reviews: 2646
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• city: Mechanicsville, Virginia

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• service: FedEx Ground / FedEx Home Delivery
• cost: $36.51
• transit: 3-7 Days
• type: Calculated
• carrier: FedEx
• class: Standard

general microscopepartzo:

• condition: Used
• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories

offer microscopepartzo:

• quantity: 1
• started: April 6, 2026
• sold: 0
• options: Ship-to-home
• availability: In Stock

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