
( Brand: Keyence ), ( Manufacturer Part Number: 49003-UF1 ), ( Part Type: Filter ), ( Country/region Of Manufacture: United States ), ( Intended Use/discipline: Biological Laboratory, Microbiological Laboratory )
The Keyence 49003-UF1 Microscope Filter Cube is a precision-engineered accessory designed for fluorescence microscopy, specifically for the excitation and emission of Enhanced Yellow Fluorescent Protein (EYFP). This filter cube is an essential tool for researchers and scientists working in the fields of cell biology, molecular biology, and life sciences.
The Keyence 49003-UF1 filter cube is a dual-band system, consisting of a DAPI filter for blue excitation (360-395 nm), a FITC filter for green excitation (450-490 nm), and an EYFP filter for yellow emission (510-550 nm). This configuration allows for simultaneous observation of multiple fluorescent markers in a single sample, aiding in the analysis of complex cellular processes.
The filter cube is meticulously crafted using high-quality materials for optimal light transmission and minimal absorption. The glass used is BK7, known for its excellent flatness and parallelism, ensuring a clear and sharp image. The filter thickness is precisely controlled to minimize chromatic aberration, further enhancing the image quality.
The Keyence 49003-UF1 Microscope Filter Cube is compatible with most inverted research microscopes and is easy to install, thanks to its standard size of 25 mm. It is designed to provide long-lasting performance, with minimal color shift or degradation over time.
In conclusion, the Keyence 49003-UF1 Microscope Filter Cube is an indispensable tool for researchers seeking to visualize and analyze EYFP-tagged proteins in living cells. Its superior quality, compatibility, and durability make it a reliable and valuable addition to any research microscope setup.
1. High Quality: The Keyence 49003-UF1 is a high-quality filter cube, known for its precision and durability. It is designed to provide accurate and reliable results in fluorescence microscopy.
2. Compatibility: This filter cube is specifically designed for EYFP (Enhanced Yellow Fluorescent Protein), making it an ideal choice for researchers working with this particular protein.
3. Bright Fluorescence: The filter cube is designed to provide bright fluorescence, ensuring that the sample is well-illuminated and the details are clear.
4. Dichroic Mirror: The dichroic mirror in the filter cube is designed to reflect the excitation light and transmit the emission light, reducing crosstalk between channels.
Cons:1. Cost: The Keyence 49003-UF1 is a premium product and may be more expensive compared to other filter cubes in the market.
2. Limited Compatibility: While it is designed for EYFP, it may not be compatible with other fluorescent proteins or stains.
3. Potential Requirement for Additional Accessories: Depending on the microscope setup, additional accessories such as a filter wheel or a motorized stage may be required, which can add to the overall cost.
Conclusion:The Keyence 49003-UF1 Microscope Filter Cube for EYFP is a high-quality product that offers bright fluorescence and reduced crosstalk. However, it comes at a premium price. If you are working primarily with EYFP and require high precision and durability, this filter cube could be a valuable investment. However, if budget is a concern or if you need a filter cube compatible with a variety of fluorescent proteins, you may want to consider other options in the market.
Recommendation:If you are working with EYFP and have the budget for a high-quality filter cube, the Keyence 49003-UF1 is a strong recommendation. If budget is a concern, consider comparing other filter cubes in the market that may offer similar performance at a lower cost. Always ensure compatibility with your microscope setup before making a purchase.
Key Specifications: model: 49003-UF1Label: ET EYFP Manufacturer: Keyence Filter Type: Fluorescence EYFP condition: Lightly used Included: Filter cube in protective case Not Included: Microscope, cables, or other accessories. The unit shows minimal signs of use and remains in excellent cosmetic functional condition. Suitable for fluorescence imaging systems requiring precise EYFP filter performance. This Keyence 49003-UF1 fluorescence filter cube is configured for ET EYFP Enhanced Yellow Fluorescent Protein applications.