CHROMA 49025 Nikon Et Dapi Fluorogold Longpass Microscope Dapi/fluorogold Filter

( Brand: Chroma ), ( Manufacturer Part Number: 49025 ), ( Part Type: Filter )

Review CHROMA Nikon Et Dapi/fluorogold Longpass Microscope Filter

Chroma 49025 Nikon E-DAPI/Fluorogold Longpass Microscope Filter

The Chroma 49025 Nikon E-DAPI/Fluorogold Longpass Microscope Filter is a high-quality optical filter designed for fluorescence microscopy applications. This filter set is specifically engineered to provide efficient excitation and emission for DAPI (4',6-diamidino-2-phenylindole) and Fluorogold (a gold conjugated secondary antibody) fluorescent dyes.

The E-DAPI component of the filter set is a band-pass filter that optimally transmits DAPI fluorescence between 436 nm and 480 nm, while rejecting excitation light and other wavelengths. This ensures that the DAPI signal is clear and distinct, allowing for accurate imaging of DNA in fixed cells and tissues.

The Fluorogold longpass filter, on the other hand, transmits light with wavelengths longer than 550 nm, effectively capturing the gold conjugate's fluorescence. This filter is essential for visualizing secondary antibodies labeled with gold, providing a strong and contrasting signal for enhanced imaging.

The Chroma 49025 filter set is designed to fit Nikon microscopes and is manufactured to the highest standards of quality and precision. It is made with Schott BG39 glass for superior optical clarity and durability. The filters are also coated with Chroma's proprietary HQ (High Quality) anti-reflection coating, which minimizes light loss and reduces ghosting and flare.

In summary, the Chroma 49025 Nikon E-DAPI/Fluorogold Longpass Microscope Filter set is an indispensable tool for researchers and microscopists working with DAPI and Fluorogold stains. Its optimized transmission spectrum, high-quality materials, and robust construction ensure accurate and vibrant imaging results.

Analysis of Chroma 49025 Nikon ET DAPI/Fluorogold Longpass Microscope Filter

1. Dual Band-Pass Filter: The Chroma 49025 is a dual filter designed for excitation at 400-440 nm (DAPI) and 520-600 nm (Fluorogold), making it suitable for dual-labeling experiments.

2. Longpass Filter: The longpass feature allows for the rejection of wavelengths shorter than 510 nm, reducing the interference of excitation light with the detection of fluorescence signals.

3. Nikon Compatibility: This filter is specifically designed for compatibility with Nikon microscopes, ensuring a proper fit and optimal performance.

4. High Quality and Durability: Chroma is known for producing high-quality filters that can withstand regular use in a laboratory setting.

1. Limited Excitation Range: While the dual band-pass feature is beneficial for certain experiments, it may not cater to every fluorescent label and its specific excitation requirements.

2. Cost: As with many specialized microscope filters, the Chroma 49025 may be more expensive compared to general-purpose filters.

3. Potential Compatibility Issues: While designed for Nikon microscopes, there may be instances where it does not work perfectly with specific models or configurations.

The Chroma 49025 Nikon ET DAPI/Fluorogold Longpass Microscope Filter is a high-quality, specialized filter that can be highly beneficial for dual-labeling experiments involving DAPI and Fluorogold. However, it's important to consider whether the dual band-pass range matches the specific excitation requirements of your experiments and whether the cost aligns with your budget. If these parameters are met, this filter could be a valuable addition to your lab equipment.

If you frequently perform dual-labeling experiments using DAPI and Fluorogold, and you own a Nikon microscope, the Chroma 49025 microscope filter would likely serve you well. However, if you have different labeling needs or use a microscope brand other than Nikon, it may be worth exploring other filter options to ensure compatibility and suitability for your specific needs.

Details:

I looked up the number 49025 and its stated to be ET - DAPI/FluoroGold Longpass The filter is in excellent condition. Nikon Chroma 49025, The filter is in excellent condition.

part #: 49025 price

• $129.00

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• brand: Chroma
• mpn: 49025
• part type: Filter

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• condition: Used
• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories

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