
( Brand: Nikon ), ( Manufacturer Part Number: 96107M ), ( Part Type: Filter )
The Nikon 96107M Fluorescence Filter Cube (31001) is a meticulously crafted optical accessory designed for advanced fluorescence microscopy applications. This filter cube caters specifically to the excitation and emission spectra of Fluorescein Isothiocyanate (FITC) and Green Fluorescent Protein (GFP), making it an ideal tool for researchers working with these popular fluorescent tags.
The B-2E/C FITC/GFP Degradation filter cube is engineered with a thoughtful combination of optical components. The excitation filter effectively isolates the blue light (450-490 nm) needed to excite FITC and GFP. The dichroic mirror then directs the emitted green light (510-550 nm) to the emission filter, while reflecting the excitation light back towards the light source. The emission filter ensures only the green fluorescence is transmitted to the camera or eye-piece for observation.
This filter cube is designed to fit Nikon's B-2E and B-2C filter turrets, offering seamless compatibility with a wide range of Nikon microscopes. The robust construction ensures durability and stability, making it an ideal companion for repetitive and long-term fluorescence microscopy experiments.
In summary, the Nikon 96107M Fluorescence Filter Cube (31001) is a high-quality, precision-engineered tool that enables sensitive and specific detection of FITC and GFP fluorescence. Whether you're studying cellular processes, molecular interactions, or gene expression, this filter cube promises to deliver reliable, high-quality results to support your research endeavors.
1. Wide Application: This filter cube is designed for FITC (Fluorescein Isothiocyanate) and GFP (Green Fluorescent Protein) fluorescence detection, making it suitable for a wide range of biological research and microscopy applications.
2. High Quality: Nikon is a well-known brand in the field of microscopy, and this filter cube is expected to provide high-quality, reliable results.
3. Compatibility: The filter cube is designed to fit B-2E/C filter turrets, a common type of filter turret for Nikon microscopes, ensuring ease of installation.
4. Degrades Excitation Light: The filter cube is designed to degrade excitation light, reducing phototoxicity and photobleaching of the samples, thereby extending the life of your specimens.
Cons:1. Limited Excitation Range: Since this filter cube is designed specifically for FITC and GFP, it may not be suitable for other types of fluorescent dyes or proteins that require different excitation and emission wavelengths.
2. Cost: As with many specialized microscopy accessories, this filter cube may be priced higher than general-purpose filters, which could be a concern for budget-conscious researchers.
3. Potential Compatibility Issues: While the filter cube is designed to fit B-2E/C filter turrets, there could be potential compatibility issues with specific microscope models or older versions. It's always a good idea to verify compatibility before purchasing.
Conclusion:The Nikon 96107M Fluorescence Filter Cube offers a high-quality solution for researchers working with FITC and GFP, providing reliable results and reducing phototoxicity and photobleaching. However, its limited excitation range and potential compatibility issues with certain microscope models should be considered. If your research primarily focuses on FITC and GFP, and you have a compatible Nikon microscope, this filter cube could be a valuable addition to your equipment. If you require a filter cube for a different type of fluorescent dye or protein, or if you're unsure about compatibility, it may be worth exploring other options.
No degradation of filters.