
( Brand: Nikon ), ( Manufacturer Part Number: 96171M ), ( Model: 96171M R HQ ), ( Part Type: Filter )
The Nikon 961171M TRITC HQ Fluorescence Filter Cube is a high-quality optical accessory designed for enhanced fluorescence microscopy. This filter cube is specifically engineered to optimize the observation of fluorescent samples under the red/orange spectral range, making it ideal for observing Red Fluorescent Proteins (RFPs) and other TRITC-filtered specimens.
The filter cube is a three-component system consisting of an excitation filter, a dichroic mirror, and an emission filter. The excitation filter blocks unwanted light, allowing only the specific wavelength of light required to excite the fluorophore to pass through. The dichroic mirror reflects the excited light towards the sample while allowing the emitted light to pass through. The emission filter, in turn, blocks any residual excitation light and only allows the fluorescence light to reach the camera or eye piece.
This particular Nikon 96117M filter cube shows slight degradation, which may result in reduced efficiency or intensity of the fluorescence signal. However, it still offers satisfactory performance for general research or educational purposes. It is essential to note that this degradation does not affect the structural integrity or compatibility with Nikon microscopes.
In conclusion, the Nikon 96117M TRITC HQ Fluorescence Filter Cube (Slight Degradation) is a valuable tool for researchers and educators who work with TRITC-labeled specimens. Despite the degradation, it provides a cost-effective solution for fluorescence microscopy, offering excellent optical quality and compatibility with Nikon microscopes.
1. High-Quality Components: The Nikon 96171M Tritc HQ filter cube is made with premium materials, designed to provide excellent performance in fluorescence microscopy.
2. Tritc Excitation: This filter cube is optimized for excitation at 546 nm, making it suitable for observing fluorescent proteins like DsRed and CFP.
3. HQ Emission: The HQ (High Quality) designation indicates that the emission filter is designed to minimize out-of-band emissions, reducing background noise and enhancing image quality.
4. Dual-Band Barrier: The dual-band barrier filter helps protect the fluorescent sample from light leakage, preserving the signal and preventing photobleaching.
Cons:1. Degradation: The product description mentions some degree of degradation, which could potentially affect the performance of the filter cube. This could lead to decreased signal intensity, increased background noise, or both.
2. Limited Compatibility: The Nikon 96171M Tritc HQ filter cube is specifically designed for use with Nikon microscopes. If you have a different brand microscope, you may need an adapter, which could add to the cost and complexity of the setup.
3. Potential for Color Shift: Due to the degradation, there may be a slight color shift in the observed fluorescence signal, which could impact the accuracy of your results.
Conclusion:The Nikon 96171M Tritc HQ microscope filter cube offers high-quality components and is well-suited for observing fluorescent proteins like DsRed and CFP. However, the presence of degradation may lead to reduced performance, and the filter's compatibility is limited to Nikon microscopes.
Recommendation:If you are in need of a Tritc filter cube for a Nikon microscope and are willing to accept some degradation in performance, the Nikon 96171M Tritc HQ filter cube may be a suitable choice. Alternatively, you may want to consider purchasing a new filter cube from a different manufacturer to ensure optimal performance and compatibility with your microscope. Be sure to carefully inspect the filter cube upon arrival to ensure it meets your expectations for quality and performance.
Nikon TRITC R HQ fluorescence filter cube. There is some degradation on top and lamp side filters. NIKON 96171M TRITC R HQ MICROSCOPE FLUORESCENCE FILTER CUBE - SOME DEGRADATION:.