
( Brand: Chroma ), ( Manufacturer Part Number: 97324 ), ( Part Type: Filter )
The 97324 Chroma 638 TIRF Ultra Fluorescence Filter Cube, specifically designed for Nikon C-TIRF microscopy, is a high-performance optoelectronic device engineered to enhance your research capabilities in the field of biology and related sciences.
This filter cube is a crucial component that ensures optimal excitation and emission of fluorescent molecules within a specific wavelength range, in this case, 638nm. The Chroma brand, renowned for its quality and precision, guarantees excellent light transmission and minimal interference, ensuring the clarity and accuracy of your data.
The 97324 filter cube is a triple band-pass system that includes a 638/38 excitation filter, a 650dcx dichroic mirror, and a 680/40 emission filter. This design ensures efficient separation of excitation and emission light, reducing undesirable cross-talk and maximizing signal-to-noise ratio.
The 638nm excitation filter is designed to excite specific fluorophores, such as Cy5 or Alexa Fluor 647, while the 680nm emission filter allows the collection of their emitted light. The 650dcx dichroic mirror efficiently reflects the excitation light to the sample while transmitting the emitted light to the detection path.
This filter cube is designed for Total Internal Reflection Fluorescence (TIRF) microscopy, a technique that allows the visualization of fluorescent molecules near the cover glass or interface, reducing background noise and increasing signal specificity.
In summary, the 97324 Chroma 638 TIRF Ultra Fluorescence Filter Cube for Nikon C-TIRF microscopy is an essential tool for researchers who require precise and reliable excitation and emission of specific fluorophores in their TIRF microscopy experiments. Its high-quality components ensure efficient light transmission, minimal cross-talk, and excellent signal-to-noise ratio, leading to accurate and clear data.
The Nikon C-TIRF Chroma 638 nm TIRF Ultra Fluorescence Filter Cube (97324) is a specialized optical filter designed for Total Internal Reflection Fluorescence (TIRF) microscopy. Here are some pros and cons to consider before making a purchase:
Pros:1. High Quality: This filter is specifically designed for TIRF microscopy, offering excellent performance for this specific application.
2. Reduced Background Noise: The filter is designed to block out wavelengths that do not contribute to your sample's fluorescence, reducing background noise and improving the signal-to-noise ratio.
3. Enhanced Fluorescence Signal: By focusing excitation light at the coverglass-sample interface, TIRF microscopy reduces the amount of light absorbed by unspecific areas, enhancing the fluorescence signal from your sample.
4. Compatibility: This filter is designed to work with Nikon's C-TIRF system, ensuring seamless integration with your microscope setup.
Cons:1. Specialization: This filter is designed for a specific application (TIRF microscopy) and may not be as versatile as other general-purpose filters.
2. Cost: As a high-quality, specialized filter, it comes at a higher price point compared to general-purpose filters.
3. Requires Specific Setup: TIRF microscopy requires a specific setup, including a prism and an illumination system, which may not be available in all microscopes.
In conclusion, if you are engaged in TIRF microscopy and require a high-quality filter for enhanced results, the Nikon C-TIRF Chroma 638 nm TIRF Ultra Fluorescence Filter Cube (97324) could be a valuable investment. However, if you are looking for a more versatile filter or are not currently engaged in TIRF microscopy, it may be more cost-effective to consider a general-purpose filter. Always ensure that the filter is compatible with your specific microscope model before making a purchase.
Most items are shipped via USPS priority mail.
New Chroma 638 c-tirf ultra hi fluorescence filter cube for nikons open box, in original packaging.