
( Brand: Andor Technology ), ( Manufacturer Part Number: DV865AC-UV ), ( Part Type: Video Camera Card Cable Case ), ( Unit Type: Unit ), ( Country Of Origin: United Kingdom )
The **Andor DV865AC-UV iXon 897 Electron Multiplying CCD (EMCCD) Camera** paired with its **CCI-20 PCI Express Interface Card**, along with the included **high-performance cables**, represents a cutting-edge solution for researchers and scientists demanding the highest levels of sensitivity, speed, and precision in imaging applications. Designed for demanding fields such as fluorescence microscopy, live-cell imaging, quantum dot tracking, and low-light spectroscopy, this system integrates Andor s renowned **iXon EMCCD technology**, which combines ultra-low read noise (as low as 0.15 electrons RMS) with rapid frame rates and exceptional quantum efficiency particularly in the ultraviolet to near-infrared spectrum (300 nm to 1000 nm). The **DV865AC-UV model** is optimized for deep UV applications, ensuring superior performance in wavelengths below 400 nm, where traditional CCDs often struggle. Its **9.2-megapixel back-illuminated sensor** delivers high-resolution imaging with minimal dark current, even at cryogenic temperatures, while the **electron multiplying gain** (up to 1000x) amplifies weak signals without introducing significant noise, making it ideal for single-molecule studies and high-contrast imaging.
The **CCI-20 PCI Express Interface Card** serves as the backbone of this system, providing a robust, high-bandwidth connection that enables seamless data transfer at speeds exceeding **1.5 Gbps**, accommodating the camera s high frame rates and large data volumes. This card supports **Andor s Solis software**, a versatile platform for acquisition, analysis, and automation, allowing users to fine-tune exposure times, gain settings, and trigger modes with precision. The included **cables** such as the **high-speed USB 3.0 cable** (for initial setup and control) and the **low-latency PCIe cable** (for high-data-throughput applications) are engineered to minimize signal degradation, ensuring that every photon captured by the camera is transmitted with fidelity. The **coaxial and ribbon cables** are shielded to reduce electromagnetic interference, while the **fiber-optic or direct-mount options** (depending on the configuration) provide flexibility for integration with microscopes, spectrographs, or other optical systems. Together, these components form a **highly modular and scalable system**, capable of interfacing with a wide range of scientific instruments while delivering the sensitivity and reliability required for breakthrough research in biology, physics, and materials science. Whether used for dynamic cellular imaging, astrophysical spectroscopy, or advanced microscopy techniques, this setup exemplifies Andor s commitment to pushing the boundaries of optical detection technology.
### **Pros and Cons of buying a Andor DV865AC-UV Microscope Video Camera with Ixon CCD (CCI-20) PCI Card and Cables**
#### **Pros**
1. **High Sensitivity and Low Light Performance**
The Ixon CCD sensor is known for its exceptional quantum efficiency, particularly in low-light conditions. This makes it ideal for fluorescence microscopy, time-lapse imaging, and applications where signal detection is critical.
2. **Fast Readout and High Frame Rates**
The DV865AC-UV supports high-speed imaging, which is beneficial for dynamic processes such as live-cell imaging, fast kinetic studies, or high-resolution video capture. The Ixon CCD can achieve frame rates up to several hundred frames per second, depending on binning and region of interest (ROI) settings.
3. **UV and Visible Light Compatibility**
The "UV" designation indicates that the camera is optimized for ultraviolet imaging, expanding its utility for applications like fluorescence microscopy with UV excitation, protein labeling, or DNA/RNA studies.
4. **High Dynamic Range**
The Ixon CCD provides a wide dynamic range, allowing for the capture of both bright and dim signals in a single image. This is particularly useful in experiments where contrast variation is high.
5. **Back-Illuminated Sensor**
The back-illuminated (BI) design of the Ixon CCD reduces light absorption in the sensor, improving overall sensitivity compared to front-illuminated alternatives.
6. **Compatibility with Andor s Software Suite**
The camera integrates seamlessly with Andor s acquisition and analysis software (e.g., Solis, Fusion, or third-party tools like MetaMorph or ImageJ). This ensures ease of use for image capture, processing, and analysis.
7. **PCI Interface for High Data Transfer**
The CCI-20 PCI card provides a robust and high-speed interface for data acquisition, reducing latency and ensuring smooth operation with high-resolution imaging.
8. **Durability and Reliability**
Andor is a reputable manufacturer in scientific imaging, and their products are generally built to last with minimal maintenance requirements.
9. **Versatility in Microscopy Applications**
Suitable for a wide range of microscopy techniques, including confocal, widefield, phase contrast, and differential interference contrast (DIC), making it a versatile tool for research labs.
10. **Global Shutter Capability**
The Ixon CCD supports global shutter operation, which is advantageous for capturing fast-moving objects or avoiding motion artifacts in time-lapse studies.
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#### **Cons**
1. **High Cost**
The DV865AC-UV with the Ixon CCD, PCI card, and cables is a premium product, and its price may be prohibitive for small labs, students, or budget-conscious researchers. The total cost can easily exceed $10,000, depending on additional accessories.
2. **Complex Setup and Learning Curve**
High-end cameras like this require familiarity with microscopy techniques, software calibration, and hardware configuration. Users may need training or technical support to optimize performance, especially for advanced applications.
3. **PCI Interface Limitations**
While the PCI card is reliable, it is an older interface compared to modern options like Thunderbolt or USB 3.0. This could pose compatibility issues with newer operating systems or computers that lack PCI slots. Some users may need an additional PCIe-to-PCI adapter, adding complexity and potential latency.
4. **Power and Cooling Requirements**
The Ixon CCD sensor may require active cooling (e.g., a Peltier cooler) to maintain optimal performance and reduce thermal noise. This adds to the system s complexity and cost, as cooling units are not included by default.
5. **Bulkiness and Physical Constraints**
The PCI card and associated cables may require additional space in the computer setup, and the camera itself may not be as compact as USB or Thunderbolt alternatives. This could be a limitation in space-constrained labs.
6. **Software Dependency**
While Andor s software is robust, it may require additional licenses or third-party plugins for advanced analysis. Some users may prefer more open-source or flexible alternatives, which could limit workflow flexibility.
7. **Maintenance and Warranty Costs**
High-end cameras may incur higher maintenance costs over time, including sensor cleaning, calibration, or replacement parts. Warranty coverage may also be limited compared to consumer-grade electronics.
8. **Potential for Overkill in Some Applications**
For basic brightfield or low-resolution imaging, the DV865AC-UV may offer more capability than necessary. Researchers with simpler needs might find a lower-cost alternative (e.g., a sCMOS or CMOS camera) sufficient.
9. **Compatibility with Older Computers**
The PCI interface may not be supported by newer, lightweight, or ultrabook-style computers, requiring users to invest in a dedicated acquisition PC.
10. **Limited Consumer Support**
While Andor provides excellent technical support for scientific users, troubleshooting may take longer than with consumer electronics, especially for non-standard issues.
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### **Ending Conclusion**
The **Andor DV865AC-UV microscope video camera with Ixon CCD, CCI-20 PCI card, and cables** is a high-performance, specialized tool designed for advanced microscopy applications where sensitivity, speed, and dynamic range are critical. It excels in low-light fluorescence imaging, time-lapse studies, and UV-based experiments, making it a valuable asset for research labs with demanding imaging requirements.
However, its **high cost, complex setup, and PCI interface limitations** may not justify the investment for all users. Researchers should carefully assess their specific needs such as budget, software compatibility, and application requirements before purchasing. If the primary use cases align with the camera s strengths (e.g., high-speed fluorescence imaging, low-light detection, or UV microscopy), the DV865AC-UV is an excellent choice. For simpler or budget-friendly applications, alternative cameras (e.g., sCMOS or CMOS models) may offer comparable performance at a lower cost.
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### **Recommendation**
**Buy the Andor DV865AC-UV if:**- You require **high sensitivity, fast frame rates, and UV/fluorescence compatibility** for advanced microscopy.
- Your lab has the **budget and infrastructure** to support the camera, including a compatible PCI-equipped computer and potential cooling requirements.
- You prioritize **reliability, Andor s software ecosystem, and long-term durability** over cost savings.
- Your research involves **dynamic imaging, live-cell studies, or low-light detection**, where the Ixon CCD s performance is essential.
**Consider alternatives if:**- Your budget is limited, and you can achieve similar results with a **lower-cost sCMOS or CMOS camera** (e.g., Andor Zyla, Hamamatsu ORCA-Flash4, or Teledyne Photometrics CoolSNAP).
- You need **plug-and-play simplicity** (e.g., USB or Thunderbolt interfaces) and do not require PCI connectivity.
- Your applications are **not UV-sensitive** or do not demand the highest frame rates, in which case a more affordable camera may suffice.
For most **academic or industrial research labs** with specialized imaging needs, the DV865AC-UV remains a **strong, high-end recommendation**. However, prospective buyers should thoroughly evaluate their requirements and consult with Andor s technical support or a microscopy specialist to ensure the camera aligns with their workflow.
Purchased for a project but never used it. Will come with a Windows XP install disc and the original software discs.