SEMROCK FF01-579 Brightline Fluorescence Microscope Emission 579/34 25MM Filter CY3

( Brand: Semrock ), ( Manufacturer Part Number: FF01-579 ), ( Model: FF01-579/34-25 ), ( Part Type: Filter )

Review SEMROCK Brightline 579/34 25mm Fluorescence Microscope Emission Filter Cy3

The **Semrock FF01-579 BrightLine 579/34-25mm Fluorescence Microscope Emission Filter (CY3)** is a high-performance, precision-engineered optical filter designed to deliver exceptional clarity and efficiency in fluorescence microscopy applications, particularly for imaging Cy3-labeled samples. Engineered with Semrock s proprietary BrightLine technology, this filter combines superior spectral purity with minimal stray light transmission, ensuring crisp, artifact-free images that maximize signal-to-noise ratios. The 579/34nm bandpass design precisely isolates the emission spectrum of Cy3 fluorophores, centered at 579 nanometers with a 34nm bandwidth, allowing researchers to capture vibrant, high-contrast fluorescence signals while effectively blocking unwanted excitation light and autofluorescence from surrounding tissues or samples. The 25mm diameter makes it compatible with a wide range of microscope configurations, from standard widefield systems to advanced confocal and multiphoton setups, making it a versatile choice for both research and clinical diagnostics. Constructed from high-quality optical glass and coated with advanced multilayer dielectric thin films, this filter exhibits exceptional durability and resistance to environmental factors, ensuring long-term reliability in demanding laboratory conditions. Whether used in cell biology, neuroscience, or molecular imaging, the FF01-579 BrightLine filter enhances the precision of fluorescence detection, enabling researchers to visualize intricate biological structures with unparalleled clarity and confidence.

### **Pros and Cons of buying a Semrock FF01-579 BrightLine 579/34 25mm Fluorescence Microscope Emission Filter (CY3)**

#### **Pros**

1. **High Optical Quality** The BrightLine series from Semrock is known for its superior optical performance, with minimal autofluorescence and high transmission efficiency. This ensures clearer, more accurate fluorescence imaging, particularly for CY3 (indocarbocyanine) dyes, which are commonly used in biological labeling.

2. **Precision Bandpass Design** The 579/34 nm bandpass filter is optimized for CY3 fluorescence, allowing only the desired emission wavelengths to pass while blocking excitation light and other spectral artifacts. This reduces background noise and improves signal-to-noise ratio.

3. **Durability and Stability** Semrock filters are manufactured with high-quality materials and precise coating techniques, ensuring long-term stability and resistance to environmental factors like humidity and temperature fluctuations, which is critical for laboratory equipment.

4. **Compatibility with Common Microscopes** The 25mm diameter is a standard size for many fluorescence microscope filter cubes and filter wheels, making it easy to integrate into existing setups without requiring custom modifications.

5. **Minimal Autofluorescence** The BrightLine technology is designed to minimize autofluorescence from the filter itself, which is particularly important for long-term imaging experiments where background interference could distort results.

6. **Brand Reputation** Semrock is a well-established manufacturer in the optics industry, known for reliability and customer support. Their filters are widely trusted in research and clinical applications, which can be reassuring for buyers.

7. **Cost-Effective for High-Quality Imaging** While not the cheapest option, the filter s performance justifies its price for serious fluorescence microscopy work, where image quality directly impacts experimental outcomes.

---

#### **Cons**

1. **Higher Initial Cost** Compared to generic or lower-end filters, the Semrock FF01-579 is more expensive. This may be a barrier for budget-conscious buyers or small labs with limited funding.

2. **Potential Overkill for Basic Use** If you are conducting very simple fluorescence imaging (e.g., occasional brightfield overlays with minimal spectral requirements), a more affordable filter might suffice. The BrightLine series is optimized for high-end applications, which may not always be necessary.

3. **Limited Customization** The filter s specifications (e.g., bandwidth, center wavelength) are fixed. If your application requires a slightly different emission range, you would need to purchase a different filter, adding to the cost.

4. **Requires Proper Handling** Like all optical components, this filter must be handled with care to avoid scratches or contamination. Improper storage or cleaning can degrade performance over time.

5. **Dependence on Filter Cube Compatibility** While the 25mm size is common, you must ensure that your microscope s filter cube or filter wheel can accommodate it without misalignment. Some older or non-standard setups may require additional adapters.

6. **No Warranty on Damage from Misuse** Standard warranties typically cover manufacturing defects but not damage caused by improper use (e.g., exposure to solvents, excessive pressure, or physical impact).

---

### **Conclusion**

The **Semrock FF01-579 BrightLine 579/34 25mm emission filter** is a high-quality, reliable choice for fluorescence microscopy applications involving CY3 dyes. Its superior optical performance, durability, and minimal autofluorescence make it ideal for research labs, clinical diagnostics, and any setting where image clarity and precision are critical. However, the higher cost and potential over-specification for basic use may not justify the purchase for casual or low-budget users.

If your work involves **complex fluorescence imaging, long-term experiments, or high-resolution imaging**, this filter is a strong recommendation. For **occasional or simpler applications**, a more economical alternative (e.g., from other reputable brands like Chroma or Thorlabs) might be sufficient.

### **Final Recommendation**

- You are working with CY3-labeled samples and require optimal fluorescence imaging.

- Your budget allows for a premium optical component that will enhance the quality of your results.

- You prioritize long-term reliability and minimal maintenance issues.

- Your application does not demand the highest possible optical performance.

- You are working with a tight budget and can achieve similar results with a lower-cost filter.

- Your microscope setup has non-standard requirements that may complicate integration.

For most serious fluorescence microscopy applications, the Semrock filter is a **worthwhile investment** due to its performance and longevity.

Details:

For sale is a 25mm emission filter commonly used with Cy3 sets for fluorescence microscopy. Semrock Cy3 Brightline Emission Filter. Details: Only what is pictured included. This 579/34 is a filter with wave length at 579nm and 34nm wide band pass designated for an emission slot.

This filter is believed to be used, but in excellent condition with no flaw.

part #: ff01579 prices

• $75.00-$599.95

specifications microscopepartzo:

• brand: Semrock
• mpn: Ff01-579
• model: Ff01-579/34-25
• part type: Filter

general microscopepartzo:

• condition: Used
• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories

offer microscopepartzo:

• best offers: True
• availability: In Stock
• options: Ship-to-home
• quantity: 10
• sold: 0
• started: April 29, 2026

seller microscopepartzo:

• city: Half Moon Bay, California
• rating: 100.0%
• # reviews: 1123

payments microscopepartzo:

• wallet: Google-pay
• other
• credit card: Diners-club

shipping microscopepartzo:

• cost: $9.95
• service: USPS Priority Mail
• carrier: USPS
• class: Expedited
• type: Fixed
• transit: 3-8 Days

returns microscopepartzo:

• time: 14 Days
• policy: Returns Accepted
• paid by: Buyer
• method: Money back

Semrock FF01-579 Is Similar To:

• 23771 Semrock Nikon Microscope Brightline (38.7% similar) Nikon e2 e4 e6 e8 E10 TS1 TS100F, TE2 TE3 ME600L, L150A, some Labrador, uptight, divot series. Lot of 3 Nikon Semrock brightline gadfly filter cubes in good condition with filters...(January 30th, 2018)
• 435-40-25 Semrock Brightline Hard Coat Zero Shift Fluorescence Excitation Filter (56.4% similar) ...(April 29th, 2018)
• 605-15-25 Semrock Brightline Hard Coated Zero Shift Fluorescence Emission Filter (60.1% similar) ...(April 29th, 2018)
• 655-15-25 Semrock Brightline Hard Coated Zero Shift Fluorescence Emission Filter (60.1% similar) ...(April 29th, 2018)
• Filter Cfp-2432a-nte Cfp Semrock Cube (14.9% similar) This is a filter cube for Nikon te series microscopes such as te 20 50I, 55I, 80I, 90I, eclipse ti, Ni, and Ci. The 2432A was discontinued in 2010 , the current 2432C filter cube implements minor improvements over the a cube. The cube combines excitation, diacritic and emission filters with the ...(January 29th, 2015)
• Cy5-4040a-omf-zero Semrock Brightline (30% similar) ...(May 5th, 2017)
• Dapi-1160a-nqf-zero Semrock Nikon Microscope Brightline (34.8% similar) Nikon e2 e4 e6 e8 E10 TS1 TS100F, TE2 TE3 ME600L, L150A, some Labrador, uptight, divot series. The new Semrock model number is DAPI-1160B-NQF-ZERO list price for cube about from. Semrock brightline DAPI-1160A-NQF-ZERO gadfly filter cube in excellent condition...(December 5th, 2017)
• Dapi-1160b Semrock Olympus Dapi Fluorescence Filter Set In Cube (43.8% similar) Fits fluorescence illuminates u-IRA BX40, BX50, BX60, ix-RFC IX70 microscope BX2-URA BX41, BX51, BX61 microscopes and IX2-RFAC IX51, IX71 IX81. Filters are clear and in excellent condition. Mounted in Olympus BX2 filter cube U-MF2. Exciter: 387 11, emitter Semrock hard-coated DAPI-1160B high ...(April 4th, 2018)
• Dapi-5060b-000 Semrock Brightline Filterset For (40% similar) Semrock brightline DAPI-5060B-000 filter set for microscope, include FF409-DI02-25MMX36MM dishrag mirror, FF01 filter, FF02, clean optics like new...(January 28th, 2018)
• Dapi-5060b-omf-zero Olympus Semrock Dapi-1160a-omf-zero Brightline (26.8% similar) Used Olympus filter cube...(April 18th, 2017)
• Ff01-560-25-25 Semrock Brightline Single-band Bandpass (35% similar) ...(April 24th, 2017)