
( Brand: Semrock ), ( Manufacturer Part Number: FF01-650/SP-25 ), ( Part Type: Filter )
The Semrock FF01-650/SP-25 BrightLine 650/SP Short-Pass 650nm Edge-Blocking Fluorescence Filter is a high-quality optical filter designed for precise fluorescence applications. This filter is specifically engineered to transmit light with a wavelength shorter than 650nm, while effectively blocking light with a wavelength of 650nm and longer.
The BrightLine 650/SP filter is part of Semrock's renowned BrightLine series, which is known for its exceptional optical performance, low autofluorescence, and high reproducibility. The filter is made using Semrock's proprietary ion-beam sputtering technology, ensuring superior edge blocking and minimal stray light.
The FF01-650/SP-25 has a peak transmission at 647nm with a full width at half maximum (FWHM) of 17nm. It features a high transmission of up to 90% and a high rejection of 650nm and longer wavelengths, ensuring a sharp cut-off and minimal light leakage.
This filter is ideal for fluorescence microscopy, spectroscopy, and other applications where precise wavelength selection is crucial. Its compact size of 25mm makes it suitable for a wide range of instruments, and the high-quality glass substrate ensures long-term durability and stability.
In summary, the Semrock FF01-650/SP-25 BrightLine 650/SP Short-Pass 650nm Edge-Blocking Fluorescence Filter is a versatile and reliable optical filter that offers excellent transmission, edge blocking, and rejection properties, making it an essential tool for fluorescence research and applications.
1. High Edge Blocking: The filter effectively blocks light at wavelengths longer than 650nm, reducing unwanted background noise and enhancing the signal-to-noise ratio in fluorescence microscopy.
2. BrightLine Coating: This coating increases the transmission of the filter, resulting in a brighter and clearer signal.
3. Short-Pass Filter: This type of filter allows shorter wavelength light to pass through while blocking longer wavelengths, making it ideal for applications that require the detection of shorter wavelength fluorescent dyes.
Cons:1. Specific Application: The filter is designed for specific wavelengths (650nm) and may not be suitable for other fluorescence applications requiring different wavelengths.
2. Cost: As a high-quality, specialized filter, it may be more expensive compared to general-purpose filters.
3. Limited Vendors: The filter is available from a limited number of vendors, which may limit options for price comparison or customer service.
Conclusion: The Semrock FF01-650/SP-25 BrightLine 650/SP Short-Pass 650nm Edge Blocking Fluorescence Filter is a high-performance filter that can significantly improve the quality of fluorescence images in specific applications. Its high edge blocking, BrightLine coating, and short-pass filter properties make it an excellent choice for researchers and scientists working with 650nm fluorescent dyes. However, its specific wavelength range, higher cost, and limited vendor availability are important considerations. If your research or application requires a different wavelength range, you may want to consider other filter options.
Recommendation: If you are working with 650nm fluorescent dyes and require a high-quality filter to improve the signal-to-noise ratio in your fluorescence images, the Semrock FF01-650/SP-25 BrightLine 650/SP Short-Pass 650nm Edge Blocking Fluorescence Filter is a recommended choice. However, carefully consider your budget, the specific requirements of your project, and the availability of the filter from various vendors before making a purchase decision.
Condition: Used. Comes in non-original packaging. When it is tilted, violet's and reds disappear. Buyer pays for all shipping costs.
This item is guaranteed to be as described. Model: FF01-650/SP-25. Brightline Short-Pass Filter. Semrock BrightLine 650/SP Short-Pass 650nm Edge Blocking Fluorescence Filter.
This filter is in good cosmetic condition. Specifications. Manufacturer: Semrock. 25mm diameter.
Notes: When looking through this straight-on, it passes all colors. No other testing was done.