THORLABS M365LP1-C5 Mw Typ Collimated Led Nikon Eclipse 435 Typ. 1700

( Brand: Thorlabs ), ( Manufacturer Part Number: M365LP1-C5 ), ( Part Type: Led )

Review THORLABS 435 Mw Typ. Collimated Led Nikon Eclipse Ma

The **Thorlabs M365LP1-C5 435 nm, 435 mW (typical) Collimated LED** is a high-performance, precision-engineered light source meticulously designed for integration with the **Nikon Eclipse 1700** inverted microscope system, offering exceptional brightness, stability, and spectral purity for advanced fluorescence imaging applications. Engineered with Thorlabs signature reliability, this collimated LED emits a **435 nm (blue) wavelength** with a **typical output power of 435 milliwatts**, delivering a robust, uniform illumination ideal for exciting fluorophores such as DAPI, Hoechst, and other blue-sensitive dyes. The LED s **collimated beam output** ensures minimal divergence, providing a highly focused and consistent light distribution across the microscope s field of view, which is critical for high-resolution imaging and photostability in long-duration experiments. Its **compact, modular design** allows for seamless integration into the Eclipse 1700 s optical path, whether used as a standalone illumination source or in conjunction with other Thorlabs components like filters, dichroics, or fiber optic couplers. The device incorporates **active thermal management**, featuring an integrated heat sink and precise current regulation to maintain stable output power and spectral consistency, even under prolonged use. This ensures minimal thermal drift and spectral shifts, which are essential for maintaining accurate fluorescence excitation and reducing photobleaching in sensitive samples. Additionally, the LED s **long operational lifespan** exceeding 10,000 hours under typical conditions makes it a cost-effective, low-maintenance solution for demanding research environments. Whether used for routine fluorescence microscopy, live-cell imaging, or specialized applications such as calcium imaging or FRET (F rster Resonance Energy Transfer), the M365LP1-C5 provides a bright, reliable, and spectrally precise light source that enhances the performance of the Nikon Eclipse 1700, enabling researchers to achieve superior image quality and experimental reproducibility.

**Pros and Cons of buying a Thorlabs M365LP1-C5 (435 nm, 50 mW Typ, Collimated LED for Nikon Eclipse 1700)**

### **Pros**

1. **Brightness and Power**: The 435 nm LED provides a relatively high output (50 mW typical) compared to many standard LEDs, ensuring sufficient illumination for fluorescence applications. This is particularly useful for weak fluorophores or low-light microscopy.

2. **Collimated Output**: The collimated beam design minimizes divergence, improving uniformity and focus across the field of view. This is beneficial for consistent illumination in widefield or epifluorescence setups.

3. **Compatibility with Nikon Eclipse 1700**: Thorlabs LEDs are designed to integrate seamlessly with Nikon microscopes, often featuring standard connectors (e.g., SMA or FC) that match Nikon s illumination ports or filter cubes. The M365LP1-C5 is likely optimized for this compatibility.

4. **Long Lifespan**: LEDs generally outlast traditional mercury or halogen lamps, reducing maintenance and replacement costs over time. Thorlabs LEDs are known for durability, often lasting thousands of hours.

5. **Compact and Low Heat**: Unlike lamps, LEDs generate minimal heat, reducing thermal drift and the need for cooling systems. This makes them ideal for long imaging sessions.

6. **Specific Wavelength (435 nm)**: This wavelength is useful for exciting certain fluorophores (e.g., DAPI, some GFP variants, or blue-shifted dyes) and can be advantageous for multicolor imaging if paired with appropriate filters.

7. **Modular and Upgradable**: Thorlabs offers a range of LEDs and accessories, allowing future upgrades or replacements without major system modifications.

8. **No Mercury or UV Hazards**: Unlike mercury lamps, LEDs do not pose environmental or health risks from mercury or UV exposure, making them safer for routine use.

9. **Fast On/Off Response**: LEDs turn on and off almost instantaneously, which can be useful for dynamic imaging or shuttered illumination.

10. **Cost-Effective for Low-Intensity Needs**: While not as bright as high-power lasers, this LED is cost-effective for applications where high power is unnecessary (e.g., basic fluorescence, low-magnification imaging).

---

### **Cons**

1. **Limited Intensity for Advanced Applications**: At 50 mW, this LED may not be sufficient for high-resolution confocal microscopy, super-resolution techniques, or applications requiring high photon flux (e.g., photobleaching, advanced fluorescence recovery). For these, a laser or higher-power LED (e.g., 100 mW) would be more appropriate.

2. **Spectral Width**: LEDs have a broader emission spectrum ( 20-30 nm) compared to lasers or narrowband filters, which can lead to more background fluorescence or bleed-through if the fluorophore s excitation spectrum is not perfectly matched.

3. **No Tunability**: Unlike tunable lasers or filter wheels, this LED emits a fixed wavelength (435 nm), limiting flexibility for multi-excitation experiments without additional hardware.

4. **Potential for Non-Uniform Illumination**: While collimated, the beam profile may not be perfectly uniform across the entire field of view, especially at higher magnifications or with large apertures. This could require additional optics (e.g., homogenizers) for optimal results.

5. **Filter Cube Compatibility**: The LED s output must align with the excitation filter in your Nikon Eclipse 1700 setup. If the existing filter cube is optimized for a different wavelength (e.g., 488 nm for GFP), the LED may not be fully effective, and you may need to adjust filters or use a separate LED port.

6. **Cost Relative to Alternatives**: While cheaper than lasers or high-end lamps, a Thorlabs LED may still represent a significant investment if you already have a lamp-based system. The long-term savings on maintenance may not justify the upfront cost for casual users.

7. **Limited Cooling Requirements**: Though LEDs generate less heat, some models may still require passive cooling (e.g., heat sinks) to maintain performance over extended use. Thorlabs provides cooling solutions, but this adds complexity.

8. **Potential for Drift Over Time**: While LEDs are stable, their output can degrade slightly over time, requiring occasional calibration or replacement.

9. **No Built-in Intensity Control**: Unlike lamps with adjustable brightness, some Thorlabs LEDs may require external drivers or manual adjustments to control intensity, which can be inconvenient.

10. **Compatibility Gaps**: If your Nikon Eclipse 1700 lacks a dedicated LED port or requires adapter cables, integration may require additional purchases (e.g., Thorlabs LED driver or Nikon s LED adapter kits).

---

### **Conclusion**

The Thorlabs M365LP1-C5 is a **practical and reliable choice** for users of the Nikon Eclipse 1700 who require a **low-maintenance, bright, and safe alternative to traditional lamps** for 435 nm fluorescence imaging. It excels in applications where **moderate illumination is sufficient**, such as:

- Basic fluorescence microscopy (e.g., DAPI staining).

- Low-power multicolor imaging (if paired with appropriate filters).

- Long-term imaging sessions where heat and lifespan are concerns.

- Educational or research settings where cost-effectiveness and simplicity are prioritized.

- High-resolution or high-sensitivity imaging (e.g., confocal, TIRF, or single-molecule studies).

- Applications requiring precise wavelength control or tunability.

- Systems where the existing filter cube is not optimized for 435 nm excitation.

If your primary need is **bright, stable, and safe illumination for standard fluorescence**, this LED is a **strong recommendation**. If you anticipate **future upgrades to higher-resolution techniques or multi-wavelength imaging**, consider pairing it with a **laser or higher-power LED** (e.g., Thorlabs M470LP1-C or similar) or investing in a **filter wheel** for flexibility.

---

### **Recommendation**

- You need a **reliable 435 nm light source** for basic fluorescence on your Nikon Eclipse 1700.

- You want to **replace a halogen or mercury lamp** for lower heat, longer lifespan, and safety.

- Your budget allows for a **dedicated LED illumination path** (or you can modify your setup accordingly).

- You are working with **fluorophores excited at 435 nm** (e.g., DAPI, some cyan fluorophores) and do not require higher power.

- You need **higher intensity** (e.g., for confocal or photobleaching), in which case a **laser (e.g., 445 nm diode laser)** or **higher-power LED (e.g., 100 mW)** would be better.

- Your setup lacks a **dedicated LED port**, and you are unwilling to modify the microscope.

- You require **multi-wavelength excitation**, which would necessitate additional LEDs or a filter wheel.

**Final Verdict:** For most **non-specialized fluorescence applications** on the Nikon Eclipse 1700, the M365LP1-C5 is a **solid, cost-effective upgrade** that enhances reliability and reduces maintenance. Pair it with the correct excitation/dichroic filters for optimal performance.

Details:

Printouts of the manual and other information also attached to this listing. Thorlabs COP5-A Collation Adapter for Nikon Eclipse Ti, AR Coating: 350 - 700 NM See drawing, attached. Cases count against me even if they are resolved. Variable voltage Power Supply that I tested this with.

See the pictures. The continuous mode is ideal for imaging with CCD cameras or photo diodes. The connected LED is controlled by one of three delectable modes: continuous, modulated, or trigger mode. LEDD1B T-Cube LED Driver, 1200 mA Max Drive Current Note that the cover over knob is missing.

When operating in modulation mode, an external control signal modulates the LED current and brightness of. In this mode the LED current is controlled by means of a single rotary control knob. Cosmetic only. Thorlabs M365LP1-C5 435 mW Typ. Collimated LED for Nikon Eclipse, 1700 mA, This is a mA.

The controls for LED current and operating mode are located on top. It is designed for use with Thorlabs collimated and mounted LED packages. Thorlabs M365LP1-C Collimated LED.

part #: m365lp1c5 prices

• $60.90-$749.99

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• brand: Thorlabs
• mpn: M365lp1-c5
• part type: Led

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