1. Microscope Parts Lab Equipment For Sale
  2. sku: 12628481460519721
  3. SEMROCK SS10158

SEMROCK SS10158 Brightline Tryptophan Fluorescence Filter Di TRP-A-000 Set 280/20 310 35

Trp-a-000 Semrock Ss10158 Brightline Tryptophan Fluorescence Filter Set 280/20 310 Di 35

( Brand: Semrock ), ( Manufacturer Part Number: SS10158 ), ( Model: TRP-A-000 ), ( Part Type: Filter ), ( Intended Use/discipline: Biological Laboratory, Microbiological Laboratory, Physical Laboratory )

Review SEMROCK Brightline Tryptophan Fluorescence Filter Set 280/20 310 Di 35

The **Semrock BrightLine TRP-A-000 Tryptophan Fluorescence Filter Set (280/20 310 Di 35)** is a high-performance, precision-engineered optical filter set meticulously designed for the selective excitation and detection of tryptophan fluorescence in biological and biochemical research. Tryptophan, one of the 20 standard amino acids, is renowned for its intrinsic fluorescence properties, making it a valuable intrinsic fluorophore for studying protein structure, folding dynamics, and conformational changes without the need for exogenous labeling. This filter set is optimized to exploit tryptophan s native emission spectrum, which peaks around 350 nanometers when excited at approximately 280 nanometers, thereby enabling sensitive and specific fluorescence imaging of proteins and other biomolecules in live cells, purified samples, or complex biological matrices.

At the core of this filter set is the **280/20 nm excitation filter**, a narrow-bandpass element engineered to isolate a precise 20 nm bandwidth centered at 280 nm, the optimal excitation wavelength for tryptophan. This filter effectively blocks stray light from other sources, minimizing autofluorescence and background noise while maximizing the signal-to-noise ratio. Following excitation, the **310 nm dichroic mirror (Di 35)** directs the emitted fluorescence toward the detection pathway while reflecting the excitation light away, ensuring clean separation between excitation and emission channels. The dichroic s sharp cutoff and high efficiency further enhance the clarity of tryptophan-derived fluorescence signals, reducing spectral overlap with other potential fluorophores or environmental interference.

The **35 nm emission filter** at the final stage of the filter set is a broad but carefully optimized bandpass filter centered around 350 nm, capturing the characteristic tryptophan emission peak with minimal loss of signal intensity. This filter is designed to exclude shorter-wavelength excitation light and longer-wavelength autofluorescence, thereby refining the detection of tryptophan-specific fluorescence. The entire filter set is constructed using Semrock s proprietary **BrightLine technology**, which combines high-quality optical coatings with precision manufacturing to deliver exceptional spectral purity, high transmission efficiency, and long-term stability. This ensures reliable performance across a wide range of imaging applications, from fluorescence microscopy and spectroscopy to flow cytometry and high-content screening.

Ideal for researchers investigating protein conformation, enzyme activity, or cellular processes involving tryptophan-containing proteins, this filter set is particularly useful in studies of protein folding, amyloid formation, and membrane dynamics. Its compact and modular design makes it compatible with a variety of fluorescence microscopes, spectrofluorometers, and other optical instruments, while its robust construction ensures durability and consistent performance under demanding experimental conditions. Whether used in academic laboratories, pharmaceutical research, or biotechnological applications, the **TRP-A-000 filter set** provides a robust and efficient solution for visualizing tryptophan fluorescence with unparalleled clarity and precision.

### **Pros and Cons of buying a Semrock SS10158 BrightLine TRP-A-000 Tryptophan Fluorescence Filter Set (280/280 nm Excitation, 310 nm Dichroic, 350 nm Emission)**

#### **Pros**

1. **High-Quality Optical Performance** Semrock is a well-known manufacturer of precision optical filters, offering high transmission efficiency and minimal autofluorescence, which is critical for sensitive fluorescence applications like tryptophan detection.

2. **Optimized for Tryptophan Fluorescence** The filter set is specifically designed for exciting tryptophan ( _ex 280 nm) and isolating its emission ( 350 nm), reducing background noise from other fluorophores or ambient light.

3. **BrightLine Technology** Semrock s BrightLine filters use proprietary coatings to minimize stray light and maximize signal-to-noise ratio, improving detection sensitivity.

4. **Compact and Durable Design** The filter cube is robust, designed for long-term use in fluorescence microscopes, spectrophotometers, or other imaging systems.

5. **Compatibility with Common Systems** The 280/20 excitation filter, 310 nm dichroic, and 350 nm emission filter combination is widely used in fluorescence spectroscopy and microscopy, making it easy to integrate into existing setups.

6. **Low Autofluorescence** The materials and coatings are engineered to minimize internal fluorescence, which is essential for low-level tryptophan detection.

7. **Good for Research Applications** Ideal for biochemical studies, protein analysis, or cellular imaging where tryptophan fluorescence is a key readout.

#### **Cons**

1. **Cost** High-quality filter sets from Semrock are more expensive than generic or lower-end alternatives, which may be a barrier for budget-conscious users.

2. **Limited Excitation Bandwidth** The 280/20 nm excitation filter has a narrow bandwidth (20 nm), which may reduce signal intensity if the sample has variable tryptophan content or if broader excitation is desired.

3. **Potential for Overlap with Other Fluorophores** Tryptophan emission ( 350 nm) can overlap with other endogenous fluorophores (e.g., NADH, tyrosine), requiring careful experimental design or additional filtering.

4. **Requires Proper Alignment** Like all filter cubes, improper installation or misalignment can degrade performance, though this is more of a setup concern than a flaw in the product itself.

5. **Not Ideal for Multi-Fluorophore Imaging** Since it is optimized solely for tryptophan, it cannot be used simultaneously with other fluorophores without modification.

---

### **Conclusion**

The **Semrock SS10158 BrightLine TRP-A-000 filter set** is a **high-performance, reliable choice** for applications requiring precise tryptophan fluorescence detection. Its strengths lie in its **optical purity, sensitivity, and compatibility with research-grade systems**, making it well-suited for biochemical and cellular studies where accuracy is critical. However, its **higher cost and narrow excitation bandwidth** may not be justified for casual or low-budget applications.

If **budget is not a constraint** and the primary goal is **high-sensitivity tryptophan imaging or spectroscopy**, this filter set is an excellent investment. For **general fluorescence work** or **multi-fluorophore imaging**, a more versatile or cost-effective alternative might be preferable.

### **Recommendation**

- **Buy if:** You need **high-quality, low-autofluorescence tryptophan detection** for research (e.g., protein folding studies, cellular imaging, or spectroscopy) and can justify the cost.

- **Consider alternatives if:** You require **broader excitation**, **multi-fluorophore compatibility**, or a **lower-cost solution** (e.g., generic filter sets from other manufacturers).

- **Pair with:** A **high-sensitivity detector** (e.g., CCD camera, photomultiplier tube) to maximize signal capture, as tryptophan fluorescence is inherently weak.

Details:

There may be dust, smudges, handling marks, coating or other surface imperfections not visible through the packaging. I do not have the specialized equipment, software, accessories, or technical expertise required to fully evaluate this item. Manufacturer:Semrock / IDEX Health Science. Labels show handling wear, marker writing, and storage marks.

Combined shipping availability depends on item size, weight, fragility, and packaging requirements. Please review all photographs carefully, as they are of the actual item being sold and form an important part. The set includes a UV excitation filter around 280nm, 310nm dishrag misapplied, and 357nm emission. Sold as untested optical surplus.

Laboratory, optical, and electronic equipment often requires specialized setup, calibration, consumables, software, or accessories to operate properly. Included in Sale. This is an incredible opportunity to obtain very specialized equipment used for scientific experiments by expert in the field. The 280/20 exciter is specified for transmission from 270 290nm, while the 357/44 emitter 335 379nm.

The filters are individually bagged and stored in a Semrock plastic case. Much of the equipment appeared to have been carefully stored and well maintained, though I do not know history or service record this specific item. Used / surplus optical filter set. Testing Condition.

This is a Semrock BrightLine TRP-A-000 fluorescence filter set, commonly associated with tryptophan and similar UV applications. This item is therefore being sold as-is. The dishrag component is specified as a 310nm single-edge misapplied, reflecting 255 295nm and transmitting 315 600nm, with 45 design angle of incidence. Certain delicate assemblies or accessories may be packaged separately within the shipping carton to reduce risk of damage during transit.

Some items can not, and should not be shipped with others.

part #: ss10158 price

  • $900.00

specifications microscopepartzo:

  • brand: Semrock
  • mpn: Ss10158
  • model: Trp-a-000
  • part type: Filter
  • intended use/discipline: Biological Laboratory, Microbiological Laboratory, Physical Laboratory

returns microscopepartzo:

  • policy: No Returns

offer microscopepartzo:

  • started: June 10, 2026
  • best offers: True
  • availability: In Stock
  • sold: 0
  • options: Ship-to-home
  • quantity: 1

shipping microscopepartzo:

  • service: USPS Priority Mail
  • carrier: USPS
  • cost: $8.07
  • transit: 5-13 Days
  • type: Fixed
  • class: Expedited

seller microscopepartzo:

  • # reviews: 260
  • city: Gypsum, Colorado
  • rating: 0.0%

payments microscopepartzo:

  • wallet: Apple-pay
  • other
  • credit card: Diners-club

general microscopepartzo:

  • condition: Used
  • Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories
part type: filter, intended use/discipline: biological laboratory, microbiological laboratory, physical laboratory,
category: business & industrial > medical/lab equipment attachments accessories > microscope parts accessories, sku: 12628481460519721,
Semrock SS10158 Is Similar To:
microscopepartzo logo
1 offer $900.00 USD
  • Used. Offer #1 priced at $900.00 + $8.07 shipping estimate = $908.07* total. Labels show handling wear, marker writing, and storage marks Manufacturer:Semrock / IDEX Health & Science.+$8.07 shipping

Copyright © microscopepartzo.net