NIKON T510IPXRXT Chroma Fluorescence Filter Cube T510LPXRXT EX480/EM580/DM510

( Brand: Nikon ), ( Manufacturer Part Number: T510IPXRXT ), ( Part Type: Filter )

Review NIKON Chroma Custom Ex480/em580/dm510 Fluorescence Filter Cube

The **Nikon T510IPXRXT Chroma T510LPXRXT EX480/EM580/DM510 Fluorescence Filter Cube** is a high-performance, precision-engineered optical component designed for advanced fluorescence microscopy applications, offering unparalleled clarity, efficiency, and versatility in imaging biological specimens. This filter cube combines Nikon s renowned optical expertise with Chroma Technology s cutting-edge filter design to deliver exceptional spectral purity, minimal autofluorescence, and optimized light transmission critical for high-resolution fluorescence imaging in research, medical diagnostics, and pharmaceutical development. The **T510LPXRXT** dichroic mirror, a high-quality, multilayer dielectric coating, precisely separates excitation and emission light while maintaining a steep transition band to maximize signal-to-noise ratio and reduce bleed-through between channels. This ensures that only the desired fluorescence wavelengths pass through to the detector, enhancing contrast and enabling the visualization of faint or deeply embedded cellular structures with remarkable precision.

The **EX480 excitation filter** is engineered to transmit a narrow, high-intensity band centered around 480 nm, ideal for exciting fluorophores such as GFP (Green Fluorescent Protein) and its variants, as well as other common fluorochromes like FITC and Alexa Fluor 488. Its sharp cutoff edges minimize stray light, preventing excitation of autofluorescent cellular components and reducing phototoxicity, which is essential for prolonged live-cell imaging. Complementing this is the **EM580 emission filter**, which selectively transmits fluorescence emission centered around 580 nm, capturing the full spectral output of fluorophores like mCherry, Texas Red, and Cy3 while effectively blocking residual excitation light and out-of-band noise. The **DM510 dichroic mirror** further refines this process by reflecting shorter excitation wavelengths toward the sample while transmitting the longer emission wavelengths to the camera or eye piece, ensuring optimal separation without compromising light efficiency.

Built to withstand rigorous laboratory conditions, this filter cube is constructed from durable, high-quality optical glass and coated with proprietary anti-reflective and anti-scratch layers to ensure long-term stability and consistent performance. The **XRXT (Extra-Range, Extra-Thick) design** enhances durability and reduces internal reflections, making it ideal for high-power laser-based systems and long-term imaging sessions where thermal drift or mechanical stress could otherwise degrade performance. Whether used in widefield, confocal, or light-sheet microscopy setups, this filter cube integrates seamlessly with Nikon s T-series microscopes, providing researchers with a reliable tool for capturing high-contrast, high-resolution fluorescence images with minimal background interference. Its modular design also allows for easy customization and integration with other filter cubes or filter wheels, making it a versatile addition to any fluorescence imaging workflow.

### Pros and Cons of buying a Nikon T510IPXRXT with Chroma T510LPXRXT Filter Cube and Fluorescence Accessories (EX480/EM580/DM510)

The Nikon T510IPXRXT is a high-end inverted microscope designed for advanced fluorescence and brightfield imaging. It features a robust mechanical structure, precise optics, and excellent stability, making it suitable for both research and clinical applications.

The inclusion of the Chroma T510LPXRXT filter cube (likely a DAPI/FITC/TRITC/Texas Red or similar multi-channel setup) allows for high-efficiency fluorescence imaging across multiple wavelengths. This is ideal for multi-label experiments, where different fluorophores are used to stain various cellular components simultaneously.

The microscope integrates seamlessly with Nikon s advanced imaging software, which offers powerful tools for image acquisition, analysis, and processing. Features like automated focus, time-lapse imaging, and deconvolution can significantly enhance workflow efficiency.

Nikon is known for producing durable microscopes with long-term reliability. The IPXRXT designation suggests enhanced resistance to environmental factors (e.g., temperature, humidity), which is beneficial for laboratories with varying conditions.

The microscope can be equipped with additional fluorescence filter cubes (e.g., EX480/EM580/DM510 for GFP or other fluorophores) and other accessories like cameras, illuminators, or stage top incubators. This modularity allows for future upgrades to meet evolving research needs.

The T510 series is equipped with high-quality objectives (e.g., Plan Fluor, Plan Apo) that provide sharp, high-resolution images with minimal aberrations. This is critical for detailed cellular and subcellular imaging.

The microscope can be paired with high-sensitivity cameras (e.g., CMOS or sCMOS) for capturing high-quality images and videos, which is essential for dynamic biological processes or long-term imaging studies.

Suitable for applications requiring high precision, such as cell biology, neuroscience, or molecular biology. The combination of fluorescence and brightfield capabilities makes it versatile for a wide range of experiments.

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The Nikon T510IPXRXT, especially when configured with high-end fluorescence filter cubes and accessories, is a significant investment. The cost may be prohibitive for smaller labs, startups, or individual researchers without dedicated funding.

High-end microscopes require regular maintenance, calibration, and occasional servicing. This can incur additional costs over time, particularly if specialized technicians are needed for repairs or adjustments.

The advanced features of the microscope, such as automated focus, multi-channel fluorescence, and software integration, may be overwhelming for novice users. Training and familiarization with the system can take time and effort.

The microscope is a large, heavy instrument that requires a stable, vibration-free environment. Labs may need to invest in proper bench space, environmental controls (e.g., temperature, humidity), and electrical infrastructure to support the system.

While Nikon s NIS-Elements software is powerful, it can be complex and may require a learning curve. Some users may prefer simpler, more intuitive software solutions, and the cost of licensing can add to the overall expense.

Due to its size and specialized components, the microscope is not easily movable between locations. This lack of portability may be a drawback for collaborative research or fieldwork.

Microscopy technology evolves rapidly, and while the T510 is a high-end system, newer models with improved resolution (e.g., super-resolution microscopy), automation, or AI-assisted imaging may become available in the future. Investing in this system may require eventual upgrades.

Additional accessories like high-end cameras, filter cubes, or stage top incubators can further increase the total cost of ownership. These components may not be immediately necessary but could become essential for specific research applications.

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The Nikon T510IPXRXT with the Chroma T510LPXRXT filter cube and fluorescence accessories (EX480/EM580/DM510) is a robust, high-performance microscope ideal for advanced fluorescence imaging in research laboratories. Its strengths lie in its precision optics, modularity, compatibility with Nikon s software, and reliability. However, the high initial and maintenance costs, complexity for beginners, and space requirements make it less suitable for smaller labs or individual users without dedicated resources.

- Established research laboratories with funding for high-end equipment.

- Teams or individuals conducting multi-channel fluorescence imaging, such as cell biology, neuroscience, or molecular biology research.

- Users who prioritize durability, precision, and long-term reliability and can invest in training and maintenance.

- **Mid-range inverted microscopes** (e.g., Nikon Ti-E, Zeiss Axio Observer) with essential fluorescence capabilities.

- **Used or refurbished systems** from reputable vendors to reduce costs while maintaining performance.

- **Simpler fluorescence microscopes** (e.g., Olympus IX73, Leica DMi8) if advanced features like automated focus or multi-channel imaging are not critical.

- **Leasing or shared-access programs** to access high-end equipment without full ownership costs.

Ultimately, the decision should be based on budget, specific research needs, and long-term goals. If fluorescence imaging is a core requirement and funding is available, the Nikon T510IPXRXT is an excellent choice for high-quality, versatile microscopy.

Details:

Good shape, no issues. Nikon/Chroma T510LPXRXT fluorescence filter cube. Emission filter: 580. Excitation filter: 480.

NIKON CHROMA T510LPXRXT CUSTOM EX480/EM580/DM510 FLUORESCENCE FILTER CUBE. Comes as seen in the pictures.

part #: t510ipxrxt prices

• $128.00-$190.00

specifications microscopepartzo:

• brand: Nikon
• mpn: T510ipxrxt
• part type: Filter

offer microscopepartzo:

• options: Ship-to-home
• availability: In Stock
• started: May 22, 2026
• sold: 0
• quantity: 1

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• city: Mishawaka, Indiana
• rating: 100.0%
• # reviews: 4531

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• policy: Returns Accepted
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• transit: 5-10 Days

general microscopepartzo:

• condition: Used
• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories

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