NIKON CHROMA 96365 Et Tr Texas Hc Hisn Fluorescence Cube Ci Microscope C86731 Ci/ti/ni

( Brand: Nikon Chroma ), ( Manufacturer Part Number: 96365 )

Review NIKON CHROMA Et Texas Red Hc Hisn Fluorescence Cube Ci/ti/ni Microscope

The **Nikon Chroma 96365 C86731 ET TR Texas Red / HC HisN Fluorescence Cube** is a high-performance, precision-engineered optical filter set meticulously designed for advanced fluorescence microscopy applications, particularly in systems equipped with **Confocal (CI), TIRF (TI), and High-Resolution (NI)** imaging modalities. This cube is engineered to deliver exceptional spectral separation and minimal cross-talk between excitation and emission pathways, ensuring crisp, high-contrast imaging of dual-labeled samples. The **Texas Red (TR) channel**, a versatile fluorophore widely used for labeling proteins, nucleic acids, and cellular structures, is optimized for excitation within the **561 nm range**, while the **HC HisN (High-Contrast HisN)** channel leverages the **405 nm excitation** spectrum, ideal for imaging histones or other nuclear markers with high specificity. The cube s **ET (Edgepass) filter design** ensures efficient transmission of the desired excitation wavelengths while effectively blocking stray light, reducing phototoxicity, and enhancing signal-to-noise ratios in complex biological samples. Constructed with **high-quality, durable materials**, including precision-machined glass substrates and proprietary dichroic mirrors, this filter cube maintains optical integrity even under prolonged use, making it a reliable choice for long-term imaging experiments. Whether used in **confocal microscopy** for high-resolution 3D reconstructions, **TIRF microscopy** for surface-sensitive studies, or **high-resolution structured illumination** for super-resolution imaging, the **Nikon Chroma 96365** cube provides the spectral purity and flexibility required to visualize multiple fluorophores simultaneously without interference. Its compatibility with **Nikon s advanced microscope systems** ensures seamless integration, allowing researchers to push the boundaries of fluorescence imaging in cellular and molecular biology, neuroscience, and beyond.

### **Pros and Cons of buying a Nikon Chroma 96365 C86731 ET TR Texas HC HiSN Fluorescence Cube for CI/TI/NI Microscopes**

#### **Pros**

1. **High-Quality Filter Set for Texas Red and Related Dyes**

- The cube is specifically designed for Texas Red (TR) and related fluorophores, including HiSN (High Sensitivity Near-infrared) variants, ensuring optimal excitation and emission filtering for these dyes.

- Provides sharp separation between excitation and emission spectra, reducing bleed-through and improving signal clarity.

2. **Compatibility with Nikon CI/TI/NI Microscope Series**

- The cube is engineered to fit Nikon s CI (Compound Inverted), TI (Total Internal Reflection), and NI (Nikon Inverted) microscope lines, ensuring seamless integration without modification.

- Backward and forward compatibility with existing filter turret setups on these microscopes.

3. **Durability and Stability**

- Chroma s filter sets are known for their precision manufacturing and long-term stability, minimizing drift over time.

- The cube is built to withstand repeated use in laboratory environments without degradation.

4. **Enhanced Imaging Performance**

- The HiSN variant is optimized for high-sensitivity imaging, reducing background noise and improving the detection of weak fluorescence signals.

- Useful for applications requiring deep tissue imaging or low-light conditions, such as fluorescence microscopy in cell biology or neuroscience.

5. **Standardized and Reliable Performance**

- Chroma is a trusted manufacturer in the microscopy industry, with filter sets widely used in research and clinical settings.

- The cube adheres to industry standards for fluorescence imaging, ensuring reproducible results across different labs.

6. **Flexibility for Multi-Dye Applications**

- While primarily designed for Texas Red, the cube can be used in combination with other compatible fluorophores (e.g., Cy3, Alexa Fluor 594) in multi-channel imaging setups on supported Nikon microscopes.

---

#### **Cons**

1. **Limited to Specific Fluorophores**

- The cube is optimized exclusively for Texas Red and related dyes (e.g., HiSN). If your research involves other common fluorophores like FITC, DAPI, or GFP, you would need additional cubes, increasing costs.

- Not suitable for broad-spectrum imaging without supplementary filter sets.

2. **Cost**

- Chroma s high-quality filter sets are premium-priced, which may be a significant investment for labs on a tight budget.

- The cost may be justified only if Texas Red/HiSN imaging is a critical component of your workflow.

3. **Potential for Overkill in Some Applications**

- If your microscope rarely uses Texas Red or if you primarily rely on other fluorophores, purchasing this cube may not be cost-effective.

- Labs with diverse imaging needs might prefer a multi-channel cube or a turret with interchangeable filters.

4. **Compatibility Dependencies**

- While compatible with Nikon s CI/TI/NI series, ensure your specific microscope model supports the cube s physical dimensions and turret interface. Consult Nikon s documentation to confirm.

- Some older or modified microscopes may require additional adapters or adjustments.

5. **Maintenance and Replacement Risks**

- Over time, filter cubes can degrade due to exposure to light, heat, or mechanical stress, potentially requiring replacement.

- Chroma s filters are not infinitely durable, and long-term use may necessitate periodic upgrades.

6. **Learning Curve for New Users**

- If your lab is unfamiliar with Chroma s filter sets or Nikon s imaging software, there may be a learning curve in optimizing settings for Texas Red/HiSN imaging.

- Proper alignment and calibration are essential to avoid artifacts or suboptimal results.

---

### **Conclusion**

The **Nikon Chroma 96365 C86731 ET TR Texas HC HiSN Fluorescence Cube** is a specialized, high-performance filter set ideal for researchers who rely heavily on Texas Red or HiSN fluorophores in their Nikon CI/TI/NI microscopes. Its strengths lie in its precision engineering, compatibility with Nikon systems, and ability to enhance imaging sensitivity for specific applications. However, its narrow fluorophore compatibility and relatively high cost may not justify its purchase for labs with diverse or limited imaging needs.

### **Recommendation**

- Your research exclusively or primarily involves Texas Red, HiSN, or related fluorophores.

- You use a Nikon CI/TI/NI microscope and require a reliable, high-sensitivity filter set for these dyes.

- Budget constraints allow for the investment, and the cube will be used frequently enough to justify its cost.

- Your imaging workflow involves a wide range of fluorophores (e.g., FITC, GFP, DAPI), as you would need additional cubes.

- You are working with a microscope that does not support this cube s specifications.

- Your lab has limited funds and can achieve similar results with a more versatile or lower-cost alternative (e.g., a multi-channel cube or a different manufacturer s filter set).

- If budget is a concern, explore Chroma s other Texas Red cubes (e.g., ET TR Texas Red) without the HiSN variant, which may offer similar performance at a lower cost.

- For labs needing multi-channel imaging, consider a turret with interchangeable cubes (e.g., Chroma s ET or Semrock s BrightLine filters) to accommodate various fluorophores.

- If compatibility with other microscope brands is a priority, evaluate filter sets from manufacturers like Semrock, Zeiss, or Leica, which may offer broader compatibility.

Details:

Clear filters, great condition. HISN - High Signal to Noise. P/N: Nikon 96365/Chroma C86731. HC - Hard Coated.

part #: 96365 price

• $9950.00

specifications microscopepartzo:

• brand: Nikon Chroma
• mpn: 96365

shipping microscopepartzo:

• type: Fixed
• service: Standard
• class: Standard
• transit: 2-6 Days
• cost: $0.00

payments microscopepartzo:

• credit card: Visa
• other
• wallet: Google-pay

offer microscopepartzo:

• sold: 0
• quantity: 1
• availability: In Stock
• options: Ship-to-home
• started: February 4, 2026

returns microscopepartzo:

• paid by: Buyer
• time: 30 Days
• method: Money back
• policy: Returns Accepted

seller microscopepartzo:

• city: Mishawaka, Indiana
• # reviews: 4367
• rating: 100.0%

general microscopepartzo:

• Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories
• condition: Used

Nikon Chroma 96365 Is Similar To:

• Mrd00100 Nikon Eclipse Microscope Plan Apo Dic Infinity Objective (26.8% similar) Original, new MRD00100. Working distance. List price: 1, comes with original capsule for protection when stored...(March 11th, 2018)
• 0.001mm Micrometer Microscope Vernier Nikon (25.4% similar) Received as surplus manufacturer Nikon vernier model could not find one do not know much about microscopes it moves back and forth and the rod goes in and out I am unable to test any further that is why I have to sell for parts or repair I only have what is pictured if you would like more pictures ...(February 2nd, 2015)
• 0.075 Nikon Optiphot Objective Plan Lens 2.5x 210 0 Microscope (26.1% similar) Posted with mobile. Nikon m plan 5x objective used for uptight and other microscopes. Beautiful clear, clean optics, no scratches on the tube...(January 18th, 2015)
• Adaptor Lens 0.55x Relay Tv Microscope Brand Nikon mount (21.6% similar) 55X c mount adapter for Nikon e2 e4 e8 50 55, ci, ts, LV150 series MQD42055 Nikon oem LV100 LV150 L200 300MM 800 400 microscope...(January 4th, 2015)
• 0.8-3.5x Stereo Zoom Sell Body Priced Microscope Smz-445 Nikon 45 Whs5 (24.5% similar) 5x 45 body great deal new in box priced to sell. Nikon DMZ 445 zoom stereo microscope...(January 21st, 2015)
• 0.27x Nikon Objective Lens For Microscope (40.9% similar) No manuals,no standard power cords, even if it appears in the photo, accessories, software etc are included unless pictured and noted. You can also after a sale if have return issues or any other concerns. All equipment is sold-as-is. All statements regarding products and their configurations are ...(February 9th, 2017)
• 021659 Nikon Binocular Teaching Microscope Head (39.1% similar) Nikon binocular teaching microscope head...(December 3rd, 2018)
• 032043 And 032061 Nikon Double Trinocular Head For Microscope Good Cosmetic Condition (33% similar) As shown in the photos. Shipping: if there is a shipping cost shown in the offer, it for continental us 48 states. Please look closely at the pictures. The lens appear to be scratch free. This item is in good cosmetic condition. Unable to test due lack of necessary equipment needed. Item is sold as ...(May 23rd, 2018)
• 05labonikon Nikon Labophot Microscope Camera Kit Lens Dx Apophot Opti Alp (25.6% similar) A Nikon t2 bayonet and to c mount with. 5x lens for SLR or milch cameras. Camera model's adapter attached to Nikon with an AP's-c sensors in particular the DX. This allows you to use. See example pictures below. 2 no adapter used ,30, and 30. If your microscope has this tube inside you can use the ...(April 9th, 2018)
• Adapter 0.5x Leica 1.0xlens Leitz Microscope 3 2 Camera Iso38 Olympus Nikon M4 (30.8% similar) Assembly pieces minus. The adapter uses a standard ISO 38MM x c mount LTZ Leila wild ISO 38. The device is designed to insert into any microscope which contains a 38MM photo port bottom lower portion of picture. 0x port adapter see picture below for separate parts. 0x with f mount. 54 5, 31, 6, 49 ...(February 13th, 2015)
• 1-25p-ahlx-4 Arcturus Labs Iphone Microscope Adapter For Photoport Leitz Nikon Olympus (21.5% similar) They clamp on to the viewing ocular but this prevents microscope from being used with your camera in position these inclined oculars. This one unit is for the iPhone 4 or 4S. Be sure it is 1x without an optic. Also works on microscopes usually inverted type with side camera ports and 1x c-mount ...(April 30th, 2018)