1. Microscope Parts Lab Equipment For Sale
  2. sku: 12511905165932602
  3. NIKON MRF00101

NIKON Fluor Microscope Objective Lens 10X/0.50 MRF00101S

Mrf00101 Nikon S Fluor 10x/0.50 Microscope Objective Lens

( Brand: Nikon ), ( Manufacturer Part Number: MRF00101 ), ( Part Type: Len ), ( Country Of Origin: United States ), ( Intended Use/discipline: Biological Laboratory )

Review NIKON Mrf00101s Fluor 10x/0.50 Microscope Objective Lens

The **Nikon MRF00101 Fluor 10x/0.50 microscope objective lens** is a precision-engineered optical component designed to elevate the performance of fluorescence microscopy, delivering exceptional clarity, contrast, and resolution for a wide range of biological and scientific applications. Part of Nikon s renowned **Fluor series**, this objective lens is meticulously crafted to optimize light transmission and minimize aberrations, ensuring that even the faintest fluorescent signals are captured with remarkable fidelity. With a **10x magnification** and a **numerical aperture (NA) of 0.50**, it strikes an ideal balance between field of view and resolving power, making it well-suited for medium-throughput imaging tasks such as cell culture analysis, tissue section examination, and fluorescent protein tagging studies. The lens features a **working distance of 3.5 mm**, allowing for versatile sample manipulation while maintaining high-quality imaging, and its **infinity-corrected optical design** ensures compatibility with modern microscope systems equipped with tube lenses and advanced imaging sensors. Constructed with **high-index glass elements and anti-reflective coatings**, the Fluor 10x objective minimizes chromatic and spherical aberrations, enhancing color accuracy and sharpness across the visible spectrum, particularly in the blue and green fluorescence ranges. Its robust **metallic housing** and durable construction provide stability during prolonged use, while the **parfocal design** ensures seamless switching between objectives without compromising focus alignment. Whether used in research laboratories, clinical diagnostics, or educational settings, this objective lens exemplifies Nikon s commitment to optical excellence, offering researchers a reliable and high-performance tool for unlocking the intricate details of fluorescently labeled specimens with precision and ease.

The Nikon MRF00101S Fluor 10x/0.50 microscope objective lens is a versatile and widely used lens in fluorescence microscopy. Below is a detailed breakdown of its pros and cons, followed by a conclusion and recommendation.

---

**Pros:**

1. **Brightness and Resolution**: The 10x magnification with a numerical aperture (NA) of 0.50 provides a good balance between field of view and resolution. This makes it suitable for observing larger samples or lower magnification fluorescence imaging where fine details are not critical.

2. **Fluorite Glass**: The lens is made with Nikon s Fluorite glass, which minimizes chromatic aberration and spherical aberration. This ensures sharper images and better color fidelity, particularly important in fluorescence microscopy where multiple fluorophores may be used.

3. **Compatibility**: The MRF00101S is designed for Nikon s Infinity Optical System, which is widely used in modern fluorescence microscopes. It is also compatible with other systems that support 25.4 mm (1-inch) objective lenses, though parfocality and alignment may vary depending on the microscope setup.

4. **Durability and Quality**: Nikon is known for producing high-quality optics with robust construction. The lens is likely to be durable and resistant to environmental factors like dust and moisture, which is important for long-term use in laboratory settings.

5. **Versatility**: The 10x magnification is a common working magnification for many applications, including cell biology, histology, and general fluorescence imaging. It allows for a broad field of view while still providing sufficient detail for most tasks.

6. **Correction Collar**: The lens includes a correction collar, which can be adjusted to compensate for differences in cover slip thickness (typically 0.13 0.17 mm for fluorescence objectives). This feature enhances image clarity across different sample preparations.

7. **Long Working Distance**: The 10x/0.50 objective generally offers a longer working distance compared to higher NA objectives, which can be advantageous for live-cell imaging or samples that are difficult to access.

8. **Compatibility with Common Fluorophores**: The lens is optimized for fluorescence imaging, making it suitable for a wide range of fluorophores, including GFP, RFP, and other common dyes used in biological research.

---

**Cons:**

1. **Lower Numerical Aperture**: An NA of 0.50 is relatively low compared to higher NA objectives (e.g., 1.25 or 1.4). This means the lens will not provide the same level of resolution or depth of field as higher NA objectives. For fine structural details or high-resolution imaging, a higher NA lens would be preferable.

2. **Limited Depth of Field**: Lower NA objectives have a shallower depth of field, which can make it more challenging to focus on thicker samples or three-dimensional structures without losing clarity.

3. **Cost**: While not excessively expensive for a Nikon objective, the price may still be a consideration for laboratories or individuals on a tight budget. Higher NA objectives or other specialized lenses may offer better performance for specific applications but at a higher cost.

4. **Potential for Aberrations at Higher Magnifications**: Although the Fluorite glass reduces aberrations, very thick or highly refractive samples (e.g., certain types of tissue sections) may still introduce some distortion or color fringing, particularly at the edges of the field of view.

5. **Compatibility Issues**: While the lens is compatible with many systems, it may not be parfocal with every microscope setup. Users may need to adjust the focus or alignment manually, which can be time-consuming.

6. **Maintenance**: Like all high-quality optics, this lens requires careful handling to avoid scratches or dust accumulation. Cleaning should be done with appropriate tools (e.g., lens paper and cleaning solutions) to preserve its performance.

---

**Conclusion:**

The Nikon MRF00101S Fluor 10x/0.50 objective lens is an excellent choice for general fluorescence microscopy applications where a balance between field of view, brightness, and resolution is required. Its Fluorite glass construction ensures high optical quality, and its compatibility with Nikon s Infinity system makes it a reliable option for many laboratories. However, if your work requires higher resolution, greater depth of field, or imaging of very thick samples, a higher NA objective (e.g., 20x or 40x) may be more suitable. The lens is particularly well-suited for routine imaging tasks, such as observing cell cultures, tissue sections, or samples labeled with common fluorophores.

---

**Recommendation:** Purchase the Nikon MRF00101S Fluor 10x/0.50 objective lens if:

- You are working with fluorescence microscopy and need a versatile, high-quality lens for general imaging.

- Your samples are relatively thin (e.g., cell monolayers, thin tissue sections) and do not require ultra-high resolution.

- You are using a Nikon microscope with an Infinity Optical System or a compatible system that supports 1-inch objectives.

- You prioritize image clarity, color fidelity, and durability without needing the highest possible magnification or resolution.

Consider alternative objectives (e.g., higher NA lenses or different magnifications) if:

- Your work involves high-resolution imaging (e.g., detailed subcellular structures).

- You frequently image thick samples or require greater depth of field.

- You are working with very high magnification (e.g., 40x or 60x) and need to zoom in further for detailed analysis.

For most users seeking a reliable, mid-range fluorescence objective, the MRF00101S is a strong and cost-effective choice. Always ensure compatibility with your microscope setup and consider your specific imaging needs before making a purchase.

Details:

As far as I can tell, they do not affect the superb, contrast image. Also of note are some small specks in near the rear lens element. Thanks to outstanding transmission below 340nm, Super Fluor objectives are the ideal choice for like Indio-1, furs-2, and fluo-3. These objectives also have improved signal-to-noise S/N for short wave length s, due to low auto-fluorescence materials, and high N.A.s, making the fluorescence images they produce significantly sharper brighter.

Details: Only what is pictured included. Model: Dimensions: Transmittance: NA: W.D.mm: Cover glass thickness: Correction ring: Observation: CFI Super Fluor 10X 0.501.100.17BF, DF Dry/Oil, DIC, POL, FL visible light, UV. These do not appear to be opaque when viewed through the lens, but are inside lens somewhere. I tried to capture it in the last photo as they are difficult see.

They are also a great choice for photo-activation of caged compounds. For sale is a Nikon Super Fluor 10x/0.50 objective lens. CFI Super Fluor Series objective lenses provide the highest level of ultra-violet transmission, exceptionally high N.A.s, and terrific chromatic correction. With M25 mounting threads, it is compatible with all modern Nikon microscope stands.

This low mag, high NA lens if perfect for magnification fluorescence imaging and screening. The lens is in excellent condition with a small mark around the black paint surrounding front lens, but itself appears.

part #: mrf00101 price

  • $3150.00

specifications microscopepartzo:

  • brand: Nikon
  • mpn: Mrf00101
  • part type: Len
  • country of origin: United States
  • intended use/discipline: Biological Laboratory

seller microscopepartzo:

  • # reviews: 1123
  • rating: 100.0%
  • city: Half Moon Bay, California

payments microscopepartzo:

  • wallet: Apple-pay
  • credit card: Discover
  • other

shipping microscopepartzo:

  • cost: $14.95
  • transit: 4-9 Days
  • class: Expedited
  • type: Fixed
  • service: USPS Priority Mail
  • carrier: USPS

returns microscopepartzo:

  • policy: Returns Accepted
  • method: Money back
  • time: 14 Days
  • paid by: Buyer

general microscopepartzo:

  • condition: Used
  • Medical/Lab Equipment Attachments Accessories > Microscope Parts Accessories > Other Microscope Parts Accessories

offer microscopepartzo:

  • best offers: True
  • availability: In Stock
  • options: Ship-to-home
  • sold: 0
  • quantity: 1
  • started: April 29, 2026
part type: len, country of origin: united states, intended use/discipline: biological laboratory,
category: business & industrial > medical/lab equipment attachments accessories > microscope parts accessories, sku: 12511905165932602,
Nikon MRF00101 Is Similar To:
microscopepartzo logo
1 offer $3150.00 USD
  • New. Offer #1 priced at $3150.00 + $6.72 shipping estimate = $3156.72* total. 2023 manufacture date. Highest NA 10X objective available.+$6.72 shipping

Copyright © microscopepartzo.net